猪带绦虫六钩蚴超微结构的观察

目的 观察猪带绦虫六钩蚴的超微结构。 方法 猪带绦虫病患者驱虫,收集成熟虫卵。次氯酸钠法破卵壳后,等渗细胞分离液（Percoll）收集六钩蚴,人工肠液激活。热琼脂离心法制备电镜样品,透射电镜观察。 结果 猪带绦虫成熟六钩蚴呈卵圆形,（14～17）μm ×（10～13）μm,表面有不规则的突起或皱褶。六钩蚴小钩从外至内由颗粒带、纤维层和芯髓组成。成熟六钩蚴具成肌细胞、小钩生发细胞和皮层细胞等几种细胞类型。 结论 猪带绦虫六钩蚴的超微结构和缩小膜壳绦虫（Hymenolepis diminuta）六钩蚴的超微结构相似,但小钩的结构不同;猪带绦虫六钩蚴有形成上皮的双核细胞。

Observation on the Ultrastructure of Taenia solium Oncosphere

Objective To observe the ultrastructure of Taenia solium oncospheres. Methods Patients infected with Taenia solium were de-wormed by decoction arecae and pumpkin seeds to get the mature proglottids and collect eggs. The eggs were treated with sodium hypochlorite to break the eggshells. Oncospheres were collected in Percoll （iso-osmotic solution）, and activated with artificial intestinal juice. The specimens were prepared with hot agar centrifugation for ultra-thin sections and observed with transmission electron microscopy（TEM）. Results T. solium oncosphere was in oval shape with a size of （14-17）μm ×（10-13）μm. There were some irregular ecphymata or plicae on its surface. The hooks were composed of outer pellet layer, the middle fibrous layer and the central core marrow. Myoblasts, hook-forming cells and cerebral cortex cells were observed in the mature oncospheres. Conclusion The ultrastructure of Taenia solium oncosphere is similar to that of Hymenolepis diminuta, with difference in hooks. There are binucleate cells which play a role in forming epithelium in the development of oncospheres.