| 硝苯地平对牙龈成纤维细胞增殖和DNA合成的影响 |
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| 引用本文： | 孙卫斌,蒋春梅,郑仕中.硝苯地平对牙龈成纤维细胞增殖和DNA合成的影响[J].中华口腔医学杂志,2001,36(3):180-182. |
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| 作者姓名： | 孙卫斌 蒋春梅 郑仕中 |
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| 作者单位： | 1. 南京医科大学口腔医学院口腔内科
2. 南京医科大学基础同位素研究室 |
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| 基金项目： | 江苏省自然科学基金资助项目（BJ99068） |
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| 摘 要： | 目的：研究钙离子通道拮抗剂硝苯地平对人牙龈成纤维细胞的影响，方法：以硝苯地平1200．0、360．0、108．0和32．4μg/L作体外培养牙龈成纤维细胞细胞计数观察，并以^3H-胸腺嘧啶脱氧核苷（^3H-TDR)细胞内掺入法评价DNA合成量。结果：细胞计数在实验的第6、7d组间差异有极显著性。主要表现为硝苯地平1200．0μg/Lt 360.0μg/L组，细胞计数明显高于低浓度组，各组间DNA合成量差异有极显著性。进一步分析差异存在于高浓组织与其他各之间。结论：高浓度硝苯地平有刺激牙龈成纤维细胞增殖的作用。
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| 关 键 词： | 牙龈增生 硝苯地平 成纤维细胞 细胞增殖 DNA |
| 修稿时间： | 2000年4月20日 |
The effects of nifedipine on proliferation and DNA synthesis of gingival fibroblasts |
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| SUN Weibin,JIANG Chunmei,ZHENG Shizhong.The effects of nifedipine on proliferation and DNA synthesis of gingival fibroblasts[J].Chinese Journal of Stomatology,2001,36(3):180-182. |
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| Authors: | SUN Weibin JIANG Chunmei ZHENG Shizhong |
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| Institution: | Department of Oral Medicine, School of Stomatology, Nanjing Medical University, Nanjing 210029, China. |
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| Abstract: | OBJECTIVE: To investigate the effects of nifedipine, a calcium channel blocker, on cellular proliferation in gingival fibroblasts in vitro. METHODS: Gingival fibroblasts were cultured and incubated with various concentrations of nifedipine (1,200.0, 360.0, 108.0 and 32.4 micrograms/L) for up to 7 days. H-TdR incorporation assay in vitro taken was used to evaluate the amount of DNA synthesis. RESULTS: Cell numbers were significantly different among these groups on sixth and seventh day, especially higher in 1,200.0 micrograms/L and 360.0 micrograms/L groups. The incorporation percentage in high concentration groups were significantly higher than those in others. CONCLUSION: Nifedipine can influent proliferation of gingival fibroblasts in high concentration. |
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| Keywords: | Gingival hyperplasia Nifedipine Fibroblasts |
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