黄精多糖对家兔动脉粥样硬化斑块的作用

目的 探讨黄精多糖对高脂血症实验兔动脉粥样硬化斑块的消退作用.方法 将40只新西兰兔分为正常对照组(饲普通饲料)、模型组(饲胆固醇饲料)、辛伐他汀组(饲胆固醇饲料+辛伐他汀)、黄精多糖组(饲胆固醇饲料+黄精多糖),分笼喂养8周.分别于实验开始时、第2周末和第8周末,采用酶法检测血清总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、脂蛋白(a)Lp(a)]水平.第8周末处死动物,光镜观察主动脉粥样硬化斑块消退情况.结果 实验前各组血脂检测指标差异无统计学意义.实验第2周末(治疗前),模型组、辛伐他汀组和黄精多糖组血清TC分别为(10.2 ±1.6)、(11.0 ±1.8)、(11.2±1.9)mmol/L,LDL-C分别为(9.85±1.65)、(9.80±1.54)、(10.08±1.88)mmol/L,Lp(a)分别为(656±106)、(700±151)、(666±111)mg/L均显著高于正常对照组(1.3±0.3)mmol/L、(0.55±0.15)mmol/L、(106±15)mg/L,P＜0.01].实验第8周末黄精多糖干预组TC、LDL-C、Lp(a)均显著低于治疗前(6.0±2.0)mmol/L vs(11.2±1.9)mmol/L、(4.25±1.35)mmol/L vs(10.08±1.88)mmol/L、(55±14)mg/Lvs(666±111)mg/L,P＜0.01],与模型组比较,黄精多糖组主动脉粥样硬化斑块基本消退.结论 一定剂量的黄精多糖(1.6 ml·kg-1·d-1)具有消退实验性高脂血症家兔主动脉粥样硬化斑块的作用.

Effect of polygona-polysaccharids on atheromatous plaque: experiment with rabbits

OBJECTIVE: To study the effect of polygona-polysaccharids on the elimination of atheromatous plaque. METHODS: Thirty adult New Zealand rabbits were fed with high-fat and cholesterol forage for 8 weeks to establish hyperlipidemia animal models and then randomly divided into 3 equal groups: model group, simvastatin group receiving simvastatin orally, and polygona-polysaccharids group receiving polygona-polysaccharids orally. Ten rabbits were fed with normal food as control forage. At weeks 0, 2, and 8 the levels of total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and lipoprotein (a) Lp (a)] were determined with enzyme method. At the end of the week 8, all rabbits were killed to observe the elimination of aorta atheromatous plaque by microscope. RESULTS: Before the experiment, there were no significant differences in the levels of serum lipids among all groups. At the end of week 2 (before treatment), the levels of serum TC of the model group, simvastatin group, and polygona-polysaccharids group were (10.2 +/- 1.6) mmol/L, (11 +/- 1.8) mmol/L, and (11.2 +/- 1.9) mmol/L respectively, the LDL-C levels of the 3 groups were (9.85 +/- 1.65) mmol/L, (9.80 +/- 1.54) mmol/L, and (10.08 +/- 1.88) mmol/L respectively, the Lp (a) levels of the 3 groups were (656 +/- 106) mmol/L, (700 +/- 151) mmol/L, and (666 +/- 111) mmol/L respectively, all significantly higher than that of the control group (1.3 +/- 0.3) mmol/L, (0.55 +/- 0.15) mmol/L, and (106 +/- 15) mg/L respectively, all P < 0.01]. At the end of week 8 (after treatment), the levels of serum TC, LDL-C, and Lp (a) of the polygona-polysaccharids group were (6.0 +/- 2.0) mmol/L, (4.25 +/- 1.35) mmol/L, and (55 +/- 14) mg/L respectively, all significantly lower than that at the end of week 2 (11.2 +/- 1.9) mmol/L, (10.08 +/- 1.88) mmol/L, and (666 +/- 111) mg/L respectively, all P < 0.01]. The aorta atheromatous plaques in the polygona-polysaccharids group were basically eliminated compared with that in the model group. CONCLUSION: Polygona-polysaccharids (1.6 ml.kg(-1).d(-1)) has the effect of eliminating aorta atheromatous plaques.