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Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase-quantitative polymerase chain reaction and immuno-quantitative polymerase chain reaction
Authors:Pinzani Pamela  Lind Kristina  Malentacchi Francesca  Nesi Gabriella  Salvianti Francesca  Villari Donata  Kubista Mikael  Pazzagli Mario  Orlando Claudio
Affiliation:a Department of Clinical Physiopathology, Clinical Biochemistry Unit, University of Florence, Italy
b Department of Chemistry and Bioscience, Chalmers University of Technology, Gothenburg, Sweden
c Department of Human Pathology and Oncology, University of Florence, Italy
d TATAA Biocenter AB, Gothenburg, Sweden
e Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic
f Urology Department, University of Florence, Italy
Abstract:
Keywords:Real time quantitative RT-PCR   Immuno-qPCR   Laser microdissection   Prostate specific antigen
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