忍冬叶中黄酮类成分的分离与鉴定

目的为忍冬(Lonicera japonicaThunb.)叶中黄酮类化学成分药效学物质基础提供依据。方法采用反复硅胶柱色谱法、Sephadex LH-20柱色谱法等对忍冬叶进行分离纯化,并通过理化常数测定和波谱分析对分离得到的化合物进行结构鉴定。结果从忍冬叶75%乙醇提取物中分离得到7个黄酮类化合物,并鉴定其结构分别为5,7,4′-三羟基-8-甲氧基黄酮(5,7,4′-trihydroxy-8-me-thoxyflavone,1)、木犀草素(luteolin,2)、苜蓿素(tricin,3)、山萘酚-7-O-β-D-吡喃葡萄糖苷(kaempferol-7-O-β-D-glucopyranoside,4)、木犀草素-7-O-β-D-吡喃葡萄糖苷(luteolin-7-O-β-D-glu-copyranoside,5)、洋芹素-7-O-β-D-吡喃葡萄糖苷(apigenin-7-O-β-D-glucopyranoside,6)、香叶木素-7-O-β-D-吡喃葡萄糖苷(diosmetin-7-O-β-D-glucopyranoside,7)。结论化合物1、4为首次从忍冬属植物中分离得到,化合物3、6、7为首次从植物忍冬中分离得到。

Isolation and identification of flavonoidal constituents from the leaves of Lonicera japonica Thunb.

Objective To study the flavonoidal constituents from the 75% ethanol extract of the leaves of Lonicera japonica Thunb. Methods The compounds were isolated by chromatography on silica gel column, Sephadex LH-20 column, and identified on the basis of physico-chemical constant and spectral analysis. Results Seven compounds were isolated and identified as 5,7,4′-trihydroxy-8-methoxyflavone (1), luteolin (2), tricin (3), kaempferol-7-O-β-D-glucopyranoside (4), luteolin-7-O-β-D-glucopyranoside (5), apigenin-7-O-β-D-glucopyranoside (6) and diosmetin-7-O-β-D-glucopyranoside (7), respectively. Conclusion Compounds 1, 4 are obtained from this genus for the first time, and compounds 3, 6, 7 are obtained from this plant for the first time.