Bcl-2与HER-2反义寡核苷酸联合转染对人舌鳞癌Tca8113细胞的干预作用

目的探讨Bcl-2与HER-2基因反义寡核苷酸(ASODN)联合转染对人舌鳞癌Tca8113细胞增殖的干预作用及其分子机制。方法实验分成正常对照组、Bcl-2正义干预组、HER-2正义干预组、Bcl-2反义干预组、HER-2 反义干预组、Bcl-2与HER-2基因反义寡核苷酸联合干预组,共6组。脂质体转染后不同时间分别进行电镜观察、 RT-PCR检测,以观察不同条件处理后各组细胞的凋亡、Bcl-2与HER-2基因表达有无差别。结果 Bcl-2与HER-2 基因反义寡核苷酸联合干预组,转染后出现凋亡小体和各期凋亡细胞;Bcl-2基因表达下降36%,HER-2基因表达下降51%。联合转染作用优于单反义基因转染作用。结论 Bcl-2与HER-2基因反义寡核苷酸联合转染能够促进肿瘤细胞凋亡并下调Bcl-2与HER-2基因的表达。

Effect on Apoptosis of Tca8113 of Interference of Bcl-2 and HER-2 Genes Antisense Oligodeoxynucleotides Transfection

Objective To investigate the interference and the mechanisms of Bcl-2 and HER-2 genes antisense oligedeoxynucleotides combined transfection in the human tongue carcinoma Tca8113 cell lines. Methods There were 6 groups in our study, normal control group, Bcl-2 sense experimental group, HER-2 sense experimental group, Bcl-2 antisense experimental group, HER-2 antisense experimental group, Bcl-2 and HER-2 genes antisense oligodeoxynucleotides combined transfection experimental group. In the different times after liposome-mediated transfection, the cell apeptosis, Bcl-2 and HER-2 expressing level were observed by RT-PCR and electronic microscope. Results According to the results of combined transfection experimental group, the apeptosis body and apeptosis cells were observed. The expression of genes were decreased statistically in both Bcl-2 and HER-2, respectively. Bcl-2 and HER-2 combined ASODN was superior to single ASODN in the intervention of tongue carcinoma. Conclusion Bcl-2 and HER-2 ASODN can effectively interfere the expression of HER-2 and Bcl-2 genes. The expression of HER-2 and Bcl-2 can be reduced, and the apoptosis of cells can be enhanced.