胰岛素和卡托普利对糖尿病大鼠肾小球系膜细胞的影响

目的　观察胰岛素和卡托普利对体外培养的肾小球系膜细胞生长的影响和白细胞介素６（ＩＬ－６）及肿瘤坏死因子（ＴＮＦ）产生情况。方法以糖尿病大鼠模型对此进行研究。结果糖尿病大鼠（ＤＭ大鼠）系膜细胞其氚-胸腺嘧啶核苷（~３Ｈ－ＴｄＲ）掺入的每分钟记数值明显高于正常大鼠。加不同浓度胰岛素时ＤＭ大鼠和正常大鼠系膜细胞~３Ｈ－ＴｄＲ掺入的记数和上清液中ＩＬ－６的含量均分别较不加胰岛素时明显升高。加不同浓度卡托普利时，ＤＭ大鼠系膜细胞~３Ｈ－ＴｄＲ掺入的记数值和上清液中ＴＮＦ含量较不加组明显减低。结论对正常大鼠系膜细胞抑制作用只有在卡托普利１０~(－３)ｍｏｌ／Ｌ时才表现出来。胰岛素促进~３Ｈ－ＴｄＲ掺入和ＩＬ－６的产生，卡托普利抑制~３Ｈ－ＴｄＲ掺入和ＴＮＦ的产生。

Effects of insulin and captopril on growth of glomerular mesangial cells in STZ - induced diabetic rats

Objective To observe the effects of insulin and captopril on growth of rnesangial cells and on pro- duction of interleukin-6 (IL-6 ) and tumor necrosis factor (TNF) in culture media of glomerular cells. Method Streptozocin induced diabetic rats (DM rats) were compared with normal wistar rats. Results The CPM value of 3 H-TdR incorporated into mesangial cells in DM rats was significantly higher than that in Wistar rats. When the culture media was added by 5 X l0-.U/ml , 5 X 10-.U/ml and 5 X 10-. U/ml of insulin , the CPM value incorporated into mesangltal cells and the concentration of IL-6 in the culture media of both DM and wistar rats were significantly higher than those of the control ( no insulin). When captopril (10-6 mol/L, 10-4mol/L, 10-3 mol/L) was added into the media in different concentration , the cpm value of a H-TdR and the concentration of TNF in DM rats were significantly lower than those of the control (no captopril) . But the CPIH value of . H-TdR in Wistar rats was lower than that in the control , which was. only seen in the concentration of 10-.M captopril. Conclusion ins could promote IH-TdR in corporation and increase the production of IL-6 , and captopril inhibite iH-TdR in corportion and decrease the preduction of TNF .