锰中毒对新生小鼠脑发育的影响

为研究锰中毒对新生小鼠脑发育的影响 ,经口服和腹腔注射联合途径给新生昆明种小鼠染锰。结果显示 :(1)高锰组小鼠体重增长明显滞后 ,脑重和脑重 体重比值显著降低 ;(2 )在Morris水迷宫的行为检测试验中 ,高锰组小鼠前 5天每天到达潜台的平均时间显著延长 ;而在第 6天的探测试验中 ,其在原潜台象限的游泳时间则明显缩短 ;(3)高锰组小鼠的脑组织蛋白质含量明显降低 ,而乙酰胆碱酯酶活性则升高 ;(4)染锰组 ,特别是高染锰组小鼠海马CA3区的胶质细胞纤维酸蛋白免疫反应强度及其阳性产物的平均相对密度均比对照组明显增高。

Effect of manganese exposure on brain development in postnatal mice

Effects of manganese (Mn) exposure on brain development in postnatal mice were studied . The results were as follows: (1) The gain of body weight, brain weight and the ratio of brain weight to body weight in the high dose Mn exposed group was significantly decreased. (2) In the Morris Water Maze Test, the average latency discovering the hidden platform was obviously increased every day during the first 5 days in the high Mn exposed group, but the latency probing the quadrant in which the hidden platform was located previously was reduced at the 6th day. (3) The protein content of brain tissue was significantly decreased in the high Mn exposed mice, but the activity of AChE in brain was decreased. (4) The immunoreactivity of glial fibrillary acid protein (GFAP) and the average relative density of GFAP positive products in hippocampus (area CA3) of both low and high Mn exposed group, especially the high dose ones, was significantly higher than that of the control group.