二脱甲氧基姜黄素对体外培养宫颈癌Hela细胞Caspase-8的影响

目的:观察二脱甲氧基姜黄素对体外培养人宫颈癌Hela细胞Caspase-8的作用。方法:MTT法检测二脱甲氧基姜黄素对体外培养人宫颈癌细胞Hela的抑制作用;分光光度计检测其对Caspase-8酶活性的影响;免疫组化法检测其对人宫颈癌细胞Hela Caspase-8蛋白表达的影响。结果:二脱甲氧基姜黄素对人宫颈癌细胞Hela有明显的抑制作用(P0.001),且呈剂量关系,与姜黄素有显著差异(P0.001);二脱甲氧基姜黄素能增强Caspase-8酶活性,增强Caspase-8蛋白的表达。结论:二脱甲氧基姜黄素能激活Caspase-8蛋白活性,这可能也是其体外抑制宫颈癌细胞Hela生长的机理之一。

Effects of Two Demethoxylation Curcumin on Caspase-8 in Hela Cells Cultured in Vitro

Objective：To observe the effects of two demethoxylation curcumin on Caspase -8 in Hela cells cultured in vitro .Methods：MTT method was used to determine the inhibition effect of two demethoxylation cur-cumin on human cervical carcinoma cell line Hela cultured in vitro;Caspase -8 activity was detected by spec-trophotometry ;Caspase-8 protein expression of human cervical carcinoma cells Hela was determined by immu-nohistochemistry assay .Results： Two demethoxylation curcumin could significantly inhibit the proliferation of human cervical carcinoma cells Hela （P〈0.01）, the effect was taken shape of dose -dependentment relation-ship, there was obvious difference to compare with curcumin （ P〈0.01）;two demethoxylation curcumin could enhance Caspase-8 activity and the expression of Caspase -8 protein in Hela cells.Conclusion：Two deme-thoxylation curcumin could activate Caspase -8 protein, which may be one of the mechanism for inhibiting the proliferation of human cervical carcinoma cells Hela .