| 透明质酸对小鼠卵丘细胞外基质的影响 |
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| 引用本文： | 吴吉文,郑蔚颖,王玮.透明质酸对小鼠卵丘细胞外基质的影响[J].福建医科大学学报,2005,39(1):33-34,40,F003. |
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| 作者姓名： | 吴吉文 郑蔚颖 王玮 |
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| 作者单位： | 1. 福建医科大学,人体解剖学与组织胚胎学系,福州,350004
2. 福建中医学院,护理学系,福州,350001 |
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| 摘 要： | 目的 观察透明质酸(HA)对小鼠卵丘细胞外基质的影响。方法 取3～4周龄的雌鼠(含野生型和bikunin基因敲除小鼠)诱导排卵，取卵丘-卵母细胞复合体(COC)，镜下计数诱导排卵后卵母细胞。免疫荧光双重染色后观察HA及血清来源的HA相关蛋白(SHAP)在卵丘细胞外基质的分布。结果 SHAP-HA复合体缺失引起卵丘细胞外基质的变化，并影响到随后的排卵。结论 SHAP-HA参与卵丘细胞外基质的构建．细胞外基质的受损引起卵母细胞功能的破坏。
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| 关 键 词： | 卵母细胞 透明质酸 细胞外基质 排卵 |
| 文章编号： | 1672-4194(2005)01-0033-03 |
Effects of Hyaluronan on the Cumulus Extracellular Matrix in Mice |
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| WU Ji-wen ,ZHENG Wei-ying ,WANG Wei.Effects of Hyaluronan on the Cumulus Extracellular Matrix in Mice[J].Journal of Fujian Medical University,2005,39(1):33-34,40,F003. |
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| Authors: | WU Ji-wen ZHENG Wei-ying WANG Wei |
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| Institution: | WU Ji-wen 1,ZHENG Wei-ying 2,WANG Wei 1 1.Division of Anatomy,Histology and Embryology,Fujian Medical University,Fuzhou 350004,China 2.Division of Nursing,Fujian Collage of Chinese Traditional Medicine,Fuzhou 350001,China |
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| Abstract: | Objective To study the effects of hyaluronan(HA) on the cumulus extracellular matrix in mice. Methods Three-to four-week-old female mice(wild-type and bikunin-null mice) were instimulated to superovulation and isolation of cumulus oocyte complex(COC). The COC were examined with microscope. The distribution of HA and serum-derived hyaluronan-associated proteins(SHAP) in the cumulus extracellular matrix detected with immunofluoresence staining method. Results SHAP-HA complex-deficiency may causes abnormality of the cumulus matrix,and subsequently affect the successful ovulation. Conclusion SHAP-HA complex is required for the construction of intercellular matrices; the function of the oocytes were reduced significantly for the malformation of the matrices. |
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| Keywords: | cumulus oocyte hyaluronan extracellular matrix ovulation |
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