| 香烟烟雾提取物诱导A-549肺上皮细胞凋亡 |
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| 引用本文： | 张季红,徐佩茹,张晓荣,常忠生.香烟烟雾提取物诱导A-549肺上皮细胞凋亡[J].新疆医科大学学报,2005,28(12):1135-1137. |
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| 作者姓名： | 张季红 徐佩茹 张晓荣 常忠生 |
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| 作者单位： | 1. 新疆医科大学第一附属医院儿科,新疆,乌鲁木齐,830054
2. 新疆米泉市中医院,新疆,米泉,831400 |
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| 摘 要： | 目的：探讨香烟烟雾提取物诱导A-549肺上皮细胞凋亡的机制。方法：以不同浓度香烟烟雾提取物作用于A-549肺上皮细胞，用碘化丙啶（PI）及Annexin V-FITC/PI双标记法检测凋亡细胞及坏死细胞。结果：50％、30％、20％、10％及5％浓度的香烟烟雾提取物处理细胞24h后，经细胞早期凋亡检测，细胞凋亡率分别达到0、31．7％、11．9％、1．6％、0．3％，细胞坏死率分别为100％、34．2％、6．2％、1．1％、0．8％。结论：一定浓度香烟烟雾提取物可诱导A-549肺上皮细胞凋亡，凋亡与坏死相伴存在，并呈剂量依赖性。
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| 关 键 词： | 香烟烟雾提取物 A-549肺上皮细胞 流式细胞术 凋亡 |
| 文章编号： | 1009-5551(2005)12-1135-03 |
| 收稿时间： | 2005-03-28 |
| 修稿时间： | 2005年3月28日 |
A study on the apoptosis of A-549 cell induced by cigarrete smoke extract with flow cytometry |
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| ZHANG Ji-hong, XU Pei-ru, ZHANG Xiao-rong.A study on the apoptosis of A-549 cell induced by cigarrete smoke extract with flow cytometry[J].Journal of Xinjiang Medical University,2005,28(12):1135-1137. |
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| Authors: | ZHANG Ji-hong XU Pei-ru ZHANG Xiao-rong |
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| Institution: | Department of Pediatrics, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China |
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| Abstract: | Objective: To study the function of cigarette smoke extract in inducing the apoptosis of A-549.Methods: The cells were treated with cigarette smoke extract in different dosages for 24 h,marked by Annexin V-FITC/PT.Cell apoptosis was assayed by flow cytometry(FCM).Results: Early stage apoptosis examination: When the cells were treated with 50%,30%,20%,10%,5% cigarette smoke extract for 24 h,their percentages of apoptosis cells were 0,31.7%,11.9%,1.6%,0.3% respectively.At the same time,the percentages of necrosis cells were 100%,34.2%,6.2%,1.1%,0.8%.Conclusion: Cigarette smoke extract in certain concentrations can induce A-549 cells apoptosis and necrosis co-exist,depending on cigarette smoke extract's affecting dosages. |
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| Keywords: | cigarette smoke extract A-549 cells flow cytometry(FCM) apoptosis |
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