应用mycalolide B初步建立斑马鱼帕金森病模型

目的 应用mycalolide B(MB)建立斑马鱼帕金森病(Parkison's disease,PD)模型,观察斑马鱼行为学、形态学及多巴胺神经元的变化并进行系统评价.方法 同亲代斑马鱼单次产卵200 ～300枚,简单随机化分为7组:对照组(0.2％ DMSO)、MB10组(10 μg/L MB)、MB20组(20 μg/L MB)、MB40组(40 μg/L MB)、MB80组(80 μg/L MB)、MB160组(160 μg/L MB)、1-甲基4-苯基-1,2,3,6-四氢吡啶(MPTP)组(200μmol/L MPTP),每组30枚胚胎,于浓度梯度下培养;统计存活率,分析行为学变化,利用激光共聚焦显微镜观察多巴胺神经元生长情况,并与MPTP组进行比较分析.结果 MB效应浓度33.4 ～ 50.3 μg/L时,成斑马鱼出现发育迟缓、脊椎畸形、心包水肿、平衡失态、震颤等;运动速率降低明显(P＜0.05);Vmat2-GFP标记的阳性细胞减少(P＜0.01);synuclein蛋白沉积(P＜0.05).结论 建立斑马鱼PD模型的MB效应浓度为33.4～50.3 μg/L;MB诱导的斑马鱼PD模型与MPTP模型相比,行为学、多巴胺神经元数量减少方面相似,且较不产生synuclein的MPTP模型更具模式学意义.

A zebrafish model of Parkinson's disease established using mycalolide B

Objective To establish a zebrafish model of Parkinson' s disease (PD) using mycalolide B (MB),and evaluate the model by observing zebrafish behavior,morphology and dopamine neurons.Methods A total of 200 ～300 parental zebrafish eggs from a single spawning were randomly divided into 7 groups:control group,MB10 group (10 μg/L),MB20 group (20 μg/L),MB40 group (40 μg/L),MB80 group (80 μg/L),MB160 group (160 μg/L) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) group (200 μmol/L),with 30 embryos in each group.The embryos were cultured under the concentration gradient,and the survival rate and behavioral changes were observed.Laser confocal microscopy was used to observe the growth of dopamine neurons.And all the results of MB treatment groups were compared with MPTP group.Results The effective concentration of MB was 33.4 ～ 50.3 μg/L,which resulted in the abnormities such as growth retardation,spinal deformity,pericardial edema,balance loss and tremor,etc.The movement rate was significantly decreased (P ＜ 0.05).There were less Vmat2-GFP positive cells (P ＜ 0.01) and more synuclein protein deposition (P ＜ 0.05).Conclusion MB at an effective concentration of 33.4 ～ 50.3 μg/L can be used to establish zebrafish PD model.The zebrafish model induced by MB are similar with the MPTP model in behaviors and fewer dopamine neurons,but better than the other one for synuclein production.