氧化苦参碱通过调节T淋巴细胞亚群抑制HBV转基因小鼠的病毒复制

目的 观察氧化苦参碱对HBV转基因小鼠病毒复制的作用,并探讨其是否与调节T淋巴细胞亚群有关.方法 选取SPF级HBV转基因雄性小鼠36只,按随机数字表法分为模型组、氧化苦参碱组及拉米夫定组,每组12只,另取12只非转基因BALB/c雄性小鼠作为对照组.各组分别给予相应的药物治疗.观察各组小鼠肝脏组织病理变化,并检测血清及肝脏乙肝表面抗原(HBsAg)、乙型肝炎病毒HBV-DNA水平、血液T淋巴细胞亚群水平以及血清细胞因子水平.结果 治疗后,与模型组比较,氧化苦参碱组与拉米夫定组血清及肝组织HBsAg、HBV-DNA水平较低(P＜0.05),而氧化苦参碱组与拉米夫定组血清及肝组织HBsAg、HBV-DNA水平差异无统计学意义(P＞0.05).与模型组比较,氧化苦参碱组与拉米夫定组血液CD3+、CD4+T淋巴细胞、CD4+/CD8+水平较高,且氧化苦参碱组＞拉米夫定组;CD8+T淋巴细胞水平较低,且氧化苦参碱组＜拉米夫定组(P＜0.05);氧化苦参碱组与拉米夫定组血清干扰素-y(IFN-γ)、白细胞介素-2(IL-2)水平较高,且氧化苦参碱组＞拉米夫定组;白细胞介素-4(IL-4)水平较低,且氧化苦参碱组＜拉米夫定组,差异均有统计学意义(P＜0.05).结论 氧化苦参碱能有效抑制HBV转基因小鼠血清及肝组织HBsAg、HBV-DNA水平,该作用可能与调节T淋巴细胞亚群水平及Th1/Th2细胞因子水平、改善免疫功能有关.

Oxymatrine inhibits viral replication in HBV transgenic mice by regulating T lymphocyte subsets

Objective To determine the inhibitory effect of oxymatrine on viral replication in HBV transgenic mice, and investigate whether this effect is associated with regulating T lymphocyte subsets.Methods Thirty-six SPF male HBV transgenic mice were randomly divided into model group, oxymatrine group and lamivudine group, with 12 rats in each group.Another 12 non-transgenic BALB/c mice served as control group.All rats were given corresponding drug treatment.Pathological changes of liver tissue were observed in each group, and the levels of hepatitis B surface antigen (HBsAg), hepatitis B virus deoxyribonucleic acid (HBV-DNA) in serum and liver, serum T lymphocyte subsets, and serum cytokine levels were detected.Results After treatment, the HBsAg and HBV-DNA levels in serum and liver were significantly lower in the oxymatrine group and lamivudine group than the model group (P ＜ 0.05), but there was no significant difference in the serum and liver levels between the oxymatrine group and lamivudine group (P ＞ 0.05).Compared with the model control group, the proportions of CD3 + and CD4 + T lymphocytes and the ratio of CD4 +/CD8 + were obviously higher, while the proportion of CD8 + T lymphocytes was quite lower in the oxymatrine group and lamivudine group, but the oxymatrine group had better above values than the lamivudine group (P ＜ 0.05).What's more, the serum levels of interferon-γ (IFN-γ) and interleukin-2 (IL-2) were higher while that of interleukin-4 (IL-4) was lower in the oxymatrine group and lamivudine group than the model group, and the oxymatrine group also had better values (P ＜ 0.05).Conclusion Oxymatrine can effectively inhibit the levels of HBsAg and HBV-DNA in serum and liver tissue of HBV transgenic mice, which may be mediated by regulating the proportions of T lymphocyte subsets and the levels of Th1/Th2 cytokines, and improving the immune function.