壳聚糖-酪蛋白磷酸肽修饰钛合金表面的成骨细胞黏附与增殖

背景：钛合金表面只有吸附某些有机分子才能使生长因子活化,促进细胞增殖,从而激活周围的骨细胞发挥作用。目的：在钛合金种植体表面制备生物活性涂层,寻找一种有效促进骨整合的方法。方法：采用多步组装方法在硅烷偶联剂修饰的钛合金表面依次接枝壳聚糖与0,5,10g/L的酪蛋白磷酸肽,形成生物活性复合涂层,以未修饰的钛合金为对照。将经过不同处理的钛合金与成骨细胞共培养,利用细胞计数法、荧光染色法、MTT比色法评价涂层的生物学性能。结果与结论：与未修饰的钛合金相比,在壳聚糖上接枝酪蛋白磷酸肽后,钛合金表面成骨细胞的早期黏附与增殖明显提高（P〈0.05）,并且随酪蛋白磷酸肽质量浓度的增大促进作用显著增强（P〈0.05）。说明制备的壳聚糖-酪蛋白磷酸肽复合涂层可以显著提高成骨细胞的功能,增强钛合金的生物学性能,有望成为有效促进骨整合的方法。

Adhesion and proliferation of osteoblasts on the surface of titanium alloy modified by chitosan-casein phosphopeptides

BACKGROUND：The surface of titanium alloy only absorbs some organic molecule to activate the growth factor and stimulate cell proliferation,thereby activating the surrounding bone cells and producing a marked effect.OBJECTIVE：To prepare a bioactive coating on the surface of the titanium alloy implant,and to evaluate one promising strategy to enhance osseointegration.METHODS：The surface of titanium alloy modified by silane coupling agent was covalently grafted with chitosan（CS）and the concentration of 0,5,10 g/L casein phosphopeptides（CPP） in turn to form composite coatings using multi-step assembly method compared with unmodified titanium alloy.The biocompatibility of the fabricated coatings was evaluated through co-culture of the titanium alloy and osteoblasts that modified through different procedures,using cell counting,fluorescence staining,and MTT colorimetric assay.RESULTS AND CONCLUSION：Compared with the titanium alloy without modification,the early adhesion and proliferation of surface of titanium alloy were significantly increased after CS covalently grafted with CPP（P 0.05）.Moreover,with the increase of CPP concentration,the promotion enhanced significantly（P 0.05）.（The osteoblasts on the surface of titanium alloy without modification displayed smooth surface,clear boundary,not fully stretched;the osteoblast cell body of the surface of titanium alloy increased significantly,rough surface,fuzzy boundaries,and fully stretched after modification by CS-CPP composite coating）.It is indicated that the prepared CS-CPP composite coating can significantly increased the function of osteoblasts,improve biological properties of titanium alloy,and expected to be an effective method to promote osseointegration.