静电纺丝纳米纤维膜作为骨骼肌组织工程支架材料的细胞相容性

背景：有报道以生物可降解的胶原盘或聚L-乳酸、聚羟基乙酸、聚L-乳酸/聚羟基乙酸共聚物等作为骨骼肌组织工程的支架材料,各有优缺点,不能完全满足骨骼肌组织工程的需要。目的：探讨静电纺丝纳米纤维膜作为骨骼肌组织工程支架材料的可行性。方法：制备7种不同组分的静电纺丝纳米纤维膜,以其浸提液为培养基培养第3代SD乳鼠成肌细胞,以含体积分数20%新生小牛血清的F12培养基培养的为对照。采用MTT法和扫描电镜检测成肌细胞在各组材料的黏附及生长情况。结果与结论：各组分静电纺丝纳米纤维膜吸光度值与对照组间差异无显著性意义（P〉0.05）。各组分静电纺丝纳米纤维膜组成肌细胞黏附率差异有显著性意义（P〈0.05）。扫描电镜与上述结果一致。含70%聚乳酸＋20%蚕丝蛋白＋10%胶原组成电纺丝纳米纤维膜组可见大量成肌细胞黏附,呈梭形,两极伸展,排列规律,效果最好。其他各组细胞少,形态不规则,似衰退期成肌细胞。提示静电纺丝纳米纤维膜无细胞毒性,对成肌细胞的增殖无影响,成肌细胞能良好地黏附;以70%聚乳酸＋20%蚕丝蛋白＋10%胶原组分效果最佳。

Cytocompatibility of electro-spinning nano-fibrous scaffolds for skeletal muscle tissue engineering

BACKGROUND：Some scholars reported that biodegradable collagen plate or poly L-lactic acid（PLLA）,poly glycolic acid（PGA）,PLLA/PGA copolymer,serve as scaffold materials of skeletal muscle tissue engineering.But these materials have their own advantages and disadvantages,and still can not fulfill the needs of skeletal muscle tissue engineering.OBJECTIVE：To study the feasibility of the electro-spinning nano-fibrous membrane as scaffolds for skeletal muscle tissue engineering.METHODS：The rat myoblasts at the third passage were cultured with extracts obtaining from different kinds of electro-spinning nano-fibrous membranes（7 groups）,with an extractant of F12 media containing 20% bovine calf serum.The adhesion and growth of myoblasts on the nano-fibrous membrane were examined with MTT and scanning electron microscopy methods.RESULTS AND CONCLUSION：The absorbance value between any two groups has no statistically significant difference compared with control group（P 0.05）,while obvious statistical differences of adhesion rate lay in different groups of biomaterials（P 0.05）.The same result lies in the scanning electron microscopy image.A great quantity of myoblasts adhered to the 70% PLA ＋ 20% silk fibroin ＋ 10% collagen group.These myoblasts are spindle-shaped,regularly arranged,and connecting to adjacent cells.In other groups,the myoblasts are less and irregular,much more like myoblasts in recession.The electro-spinning nano-fibrous membrane has no toxicity and no bad effect on the growth of myoblasts.They adhere well on the membrane,especially on the 70% PLA ＋ 20% silk fibroin ＋ 10% collagen group.