骨诱导性磷酸钙陶瓷支架在脂肪组织中构建体内组织工程骨

背景：作为体内组织工程骨的构建场所,非骨组织的选择至关重要。早期研究大多选用肌肉作为异位骨移植物的构建区域,但其位置较深,可利用面积小、手术操作复杂,不利于临床推广。目的：采用体内骨组织工程的方法,探索骨诱导性磷酸钙陶瓷支架在不同非骨组织中构建骨移植物的可行性。方法：选取家犬的背部肌肉组织和脂肪组织为构建区,分别植入骨诱导性磷酸钙陶瓷支架以构建体内组织工程骨移植物。于移植后4,8,12,16周取样进行单光子计算机断层扫描及组织学检测,观察其构建过程,比较各个观测时间内,不同构建区的骨移植物中新骨组织的形成情况,评价不同非骨构建区域对体内骨组织骨移植物形成的影响。结果与结论：骨诱导性磷酸钙陶瓷支架在肌肉组织和脂肪两处非骨组织中均可形成体内组织工程骨移植物,在构建初期,肌肉组中新骨形成的时间比脂肪组早,骨量也较多,但随着构建时间的延长,两组的新生骨量差异逐渐减小。提示,应用体内骨组织工程的方法在肌肉和脂肪组织均可构建出具有生命活性的自体骨移植物。与肌肉组织比较,脂肪组织面积宽广,位置浅表,因此在脂肪组织中构建体内组织工程骨移植物更有临床应用前景。

Osteoinductive calcium phosphate ceramics for in vivo construction of tissue engineered bone in adipose tissue

BACKGROUND：For constructing tissue engineered bone,the choice of non-osseous tissue is of great importance.Most early studies adopt the muscle as the site of constructing ectopic bone graft,but its location is deep,available area is small,the operation is complex,thus it is not conducive to clinical practice.OBJECTIVE：The study is aimed to investigate the feasibility of constructing tissue engineered bone graft with osteoinductive calcium phosphate ceramics in non-osseous tissue by means of in vivo bone tissue engineering method.METHODS：The muscle tissue and adipose tissue on the back of dogs were taken as constructed area,and implanted with osteoinductive calcium phosphate ceramics to construct in vivo tissue engineered bone graft.The specimens were harvested at 4,8,12 and 16 weeks post operation,for gross observation,single photon emission computerized tomography and histological studies.The osseous tissue formation at different times in two non-osseous sites was compared and the new bone grafts in subcutaneous fat and intramuscular implantations were evaluated.RESULTS AND CONCLUSION：The tissue engineered bone graft could be constructed not only in muscle but also in subcutaneous fat.The new bone appeared in the muscle earlier than that in the fat,the amount of new bone mass increased with time in two non-osseous tissues,and the difference between in muscle and fat decreased.Vital autogenous bone graft can be constructed in the muscle and fat tissues by means of bone tissue engineering method in vivo.Compared to that in muscle,constructing bone graft in subcutaneous fat could have brighter prospect to clinical application due to widely area and superficial location.