仙灵骨葆对骨质疏松性骨折骨痂血管形成的影响

背景：骨质疏松对骨折愈合早期的影响尚存争议,仙灵骨葆对骨质疏松性骨折愈合的影响及其机制尚有待深入研究。目的：观察骨质疏松对大鼠股骨干骨折愈合的影响,以及仙灵骨葆对骨质疏松性骨折愈合的作用。方法：将50只雌性12周龄Sprague-Dawley大鼠随机分成5组：假手术组、骨折组、去卵巢组、去卵巢＋骨折组及治疗组,后3组切除大鼠双侧卵巢制备去卵巢模型,骨折模型于去卵巢后4周制备,为股骨干中段横行骨折。治疗组在去卵巢及骨折基础上灌胃给予仙灵骨葆250mg/（kg·d）。给药3周,取去卵巢＋骨折组、骨折组和治疗组大鼠骨折侧标本行X射线摄像仪摄片后,测量其骨密度,苏木精-伊红染色观察骨痂组织的病理学改变并计数血管,免疫组织化学染色检测骨痂组织骨形态发生蛋白2的表达。结果与结论：去卵巢处理后大鼠的骨密度、计算机X射线摄像仪摄片评分显著降低（P〈0.05）,仙灵骨葆可在一定程度上提高去卵巢后骨折大鼠的骨密度及X射线摄像仪摄片评分,但差异无显著性意义（P〉0.05）;仙灵骨葆可提高去卵巢后骨折大鼠骨痂组织的血管数量（P〈0.05）,但对骨形态发生蛋白2的表达无影响。提示,去卵巢后大鼠骨折早期愈合过程延迟,仙灵骨葆可促进骨质疏松大鼠骨折愈合早期血管的形成。

Xianlinggubao promotes vascular formation in osteoporotic fracture callus

BACKGROUND：Controversy still exists in impact of osteoporosis on early process of fracture healing, and the effect and related mechanism underlying Xianlinggubao （XLGB） on osteoporotic fracture healing need further study. OBJECTIVE：To confirm the influence of osteoporosis on the healing of femoral shaft fracture and to investigate the effects of XLGB on osteoporotic fracture healing. METHODS：Fifty female 12-week old Sprague-Dawley rats were randomly divided into 5 groups with 10 animals in each group：sham-surgery, fracture, ovariectomy （OVX）, OVX ＋ fracture and XLGB. OVX was performed in bilateral sides of rats. Transverse fracture model of femoral shaft was established at 4 weeks after OVX. Rats in XLGB treatment group were treated with 250 mg/kg XLGB daily for 3 weeks following the establishment of OVX and fracture. Rats in the OVX ＋ fracture, fracture and XLGB treatment groups were harvested the femoral fracture samples for bone mineral density test by X-ray radiography. The pathological changes of callus tissues were detected with hematoxylin-eosin staining and blood vessels were counted. Immunohistochemical staining was applied to determine the expression of bone morphogenetic protein 2 in callus tissues. RESULTS AND CONCLUSION： Bone mineral density test and computed X-ray radiography score showed a significantly reduction in the rats following OVX （P 0.05）. XLGB treatment could enhance the bone mineral density and X-ray radiography score in the OVX and fracture rats, with no significant difference （P 0.05）.XLGB treatment also increased the quantity of callus tissues compared with OVX ＋ fracture and fracture groups （P 0.05）. No significant difference was observed of bone morphogenetic protein-2 expression among three groups. The ovariectomized rats show a delayed fracture healing process and XLGB could promote the early vascular formation of osteoporotic fracture healing.