骨髓间充质干细胞的分离培养与鉴定

背景：近年来骨髓间充质干细胞在组织工程、细胞移植、基因治疗等领域备受重视,得到广泛研究。目的：对骨髓间充质干细胞的生物学特性、体外分离、培养及鉴定方法及其临床学的应用进行综述。方法：应用计算机检索1995-01/2010-01CNKI数据库相关文章,检索词为＂骨髓间充质干细胞,分离,培养,鉴定＂,并限定文章语言种类为中文。同时计算机检索1995-01/2010-01PubMed数据库相关文章,检索词为＂bone marrow mesenchymal stem cells,isolation,cultivation,identification＂,并限定文章语言种类为＂English＂。共检索到文献68篇,最终纳入符合标准的文献30篇。结果与结论：骨髓间充质干细胞是存在于骨髓中的一类非造血干细胞,易于获得和分离培养,且具有多向分化及高度增殖的能力。目前研究发现它具有分化为成骨细胞、软骨细胞、脂肪细胞、心肌细胞、神经细胞以及肝细胞等多种类型细胞的潜能,已经成为重要的组织工程种子细胞。

Isolation,cultivation and identification of bone marrow mesenchymal stem cells

BACKGROUND：Bone marrow mesenchymal stem cells（BMMSCs） are currently a hot subject of research in many fields such as tissue engineering,cells transplantation and gene therapy.OBJECTIVE：To review the biological property,isolation,cultivation and clinical application of BMMSCs.METHODS：We had retrieved 30 relevant articles from CNKI and PubMed databases with the key words of ＂bone marrow mesenchymal stem cells,isolation,cultivation,identification＂ in Chinese and English,respectively,between January 1995 to January 2010 respectively.RESULTS AND CONCLUSION：BMMSCs are nonhematopoietic stem cells and easy to be harvested and cultured which show varying differentiation and high proliferation potentials.BMMSCs can differentiate into osteoblasts,chondrocytes,lipocytes,myocardial cells,nerve cells and hepatocytes,and have become an important seed cells for tissue engineering.