正畸牙移动过程中牙周组织白细胞介素8的表达

背景：白细胞介素8作为一种与骨改建相关的炎性细胞因子,参与牙周组织的改建,促进牙槽骨的吸收,是骨吸收的标志物之一。目的：观察正畸力对牙周组织改建过程中白细胞介素8表达的影响。方法：将10周龄雄性SD大鼠上颌一侧第一磨牙与上颌切牙之间安置正畸装置,并施加0.49N近中向正畸力,在加力后1,3,5,7,14d取材,进行组织形态分析法、免疫组化染色检测白细胞介素8在牙周组织中表达的阳性细胞的面密度值。结果与结论：正常大鼠牙周组织内白细胞介素8的表达呈弱阳性;随加力时间的延长大鼠牙周组织牙周膜细胞白细胞介素8表达增强,加力后5d达到高峰,之后开始下降。实验提示正畸力可引起局部牙周组织炎症及白细胞介素8的释放。正畸牙移动早期,白细胞介素8的释放可能是牙周组织早期炎症反应和牙槽骨改建的触发因素之一。

Expression of interleukin-8 in periodontal tissue during experimental tooth movement in rats

BACKGROUND：Interleukin-8 （IL-8） as a bone remodeling associated with inflammatory cytokines,involved in periodontal tissue remodeling,and promote the absorption of alveolar bone,which is one marker of bone resorption.OBJECTIVE：To observe the effects of orthodontic force on （IL-8） expression during periodontal tissue remodeling.METHODS：The orthodontic devices were placed in maxillary first molars and maxillary incisors side of 10-week-old male SD rats,and impose 0.49 N orthodontic force.The specimen was harvested at 1,3,5,7 and 14 days after operation and the surface density of IL-8 positive cells in periodontal tissues were determined by morphology analysis and immunohistochemical staining.RESULTS AND CONCLUSION：The expression of IL-8 was observed at a low level in the control group.IL-8 expression increased after tooth movement and reached a peak at 5 days,after that,it gradually declined.Orthodontic force can cause local inflammation and periodontal tissue IL-8 release.IL-8 release may be an early inflammatory reaction in periodontal tissue and alveolar bone remodeling of the trigger factor at early stage of orthodontic tooth movement.