应激动物模型构建方法及效果评价的研究进展

背景：应激与疾病的关系越来越被人们所关注，应激动物模型的建立是应激研究的基础。但在应激动物建模方面尚无统一的标准。目的：就各类应激动物模型的建立方法及建立模型后应激效果的评价进行综述，并分析模型建立及评价中存在的问题。方法：应用计算机柃索PubMed数据库（2000／2010），以“stress，animal model，mudne，evaluate”为检索词：检索重庆维普数据库（2000／2010），以“应激，动物模型”为检索词。文章所述内容需与应激动物模型的建立、应用、评价，及应激与疾病的关系等方面研究密切相关，排除重复性研究。结果与结论：共收集417篇关于应激动物模型的文献，中文115篇，英文302篇。阅读标题和摘要进行初筛，排除发表时间较早、重复及类似的研究，纳入30篇符合标准的文献。目前用于研究的应激动物模型种类繁多，但造模方法还没有统一标准，对应激的程度也没仃呵量化的指标，在应激的评价方面存在缺陷。随着神经科学、分子生物学、电生理学等的飞速发展，应激研究领域正在向分予水平深入和整体网络水平延伸。转基因技术、基因敲除模型、蛋白缺失实验等技术的发展与广泛应用，为应激机制的研究提供了新思路与新手段。

Progress addressing animal stress model construction and effect evaluation

BACKGROUND： People are paying close attention to the relationship between stress and disease. Establishment of animal stress models is the basic for stress research, which is lack of united standard. OBJECTIVE： To introduce animal model construction methods and effect evaluation, in addition, to analyze existing problems during model preparation. METHODS： A computer-based online search was performed using key words of ＂stress, animal model, murine, and evaluate＂ for documents on VIP and PuMed from 2000 to 2010. Repetitive papers were excluded. RESULTS AND CONCLUSION： A total of 417 documents were collected, including 115 in Chinese and 302 in English. By reading titles and abstract, 30 papers were included in the final analysis. Currently, various animal stress models are involved, but there is not a unified standard, and the stress degree remains lack of quantizatable index. With rapidly development of neuroscience, molecular biology, and etectrophysiology, the stress research is extended into molecular level and integrated network. The wide application of transgenic technology, gene knockout models, and protein depletion provide new approach for study of stress mechanism.