细胞共培养在组织工程研究中的应用及价值

背景：细胞共培养即最大程度地模拟机体内环境,在体外观察细胞与细胞之间的相互作用,是组织工程研究的热点。目的：总结并讨论细胞共培养研究在组织工程研究的应用及价值。方法：由第一作者用计算机检索中国期刊全文数据库（CNKI：2000/2010）和Medline数据库（2000/2010）,检索词分别为＂细胞共培养,骨髓间充质干细胞,神经干细胞,软骨细胞,组织工程＂和＂Cellsco-culture,Bone marrow stem cells,Neural stem cells,Cartilage＂,语言分别设定为中文和英文。从细胞共培养在骨髓间充值干细胞、神经干细胞和软骨细胞3方面进行总结,对细胞共培养在组织工程研究及应用方面进行介绍。结果与结论：共检索到182篇文章,按纳入和排除标准对文献进行筛选,共纳入30篇文章。结果表明大量细胞共培养的研究,都围绕着最大程度地模拟体内环境、维持体内性状,观察细胞与细胞之间相互作用,很多体外细胞共培养的研究结果和进展对于深入了解共培养有重要的指导和参考价值,但有关共培养的新方法新技术,研究结果,最新动向和研究热点但仍需进一步分析和总结。

Application and value of cells co-culture in tissue engineering

BACKGROUND：Cells co-culture is one kind of culture method which was established through some special culture technique in vitro.It can simulator organism intericur milieu in the greatest degree and can observe cell interaction in vitro.It is a hot topic in tissue engineering research.OBJECTIVE：To summarize and discuss the application and value of cells co-culture in the field of tissue engineering.METHODS：First author searched literatures from CNKI and Medline database from 2000 to 2010 using of ＂cells co-culture;bone marrow stem cells;neural stem cells;cartilage＂ both in Chinese or English.Co-culture cells were summarized from bone marrow stem cells,neural stem cells and cartilage cells in this paper.The application and value of cells co-culture in tissue engineering were also introduced.RESULTS AND CONCLUSION：A total of 182 papers were searched and 30 papers were selected.The results show that most researches of co-culture are to simulation surroundings in vivo,to maintain body character,to observe cell interaction between cells to the greatest extent.These results have significant and reference value for us;however,we should further analyze and summarize the new method and technology,pay more attention to the latest trends and hotspot of cells co-culture.