密度梯度离心法体外培养骨髓间充质干细胞的分化能力

背景：骨髓间充质干细胞具有多向分化能力,是目前极具潜力的种子细胞及基因治疗的靶细胞。目的：体外培养兔骨髓来源间充质干细胞,了解其生物学特性。方法：采用密度梯度离心法培养兔骨髓间充质干细胞,对细胞的形态及生长特性进行观察,应用流式细胞仪检测细胞表面抗原CD29、CD34、CD44、CD45、CD166、HLA-DR表达,并进行相关生物学特性鉴定。结果与结论：密度梯度离心法能分离培养出纯度较高的骨髓间充质干细胞,流式细胞仪检测骨髓间充质干细胞表达CD29、CD44、CD166,不表达CD34、CD45、HLA-DR。在适当的条件下能够诱导分化成为成骨细胞、软骨细胞等。

Differentiation ability of bone marrow mesenchymal stem cells cultured by density gradient centrifugation in vitro

BACKGROUND：Bone marrow mesenchymal stem cells（BMSCs） are capable of multiple directional differentiation,and are potential seed cells and target cells for gene therapy.OBJECTIVE：To observe in vitro cultured rabbit BMSCs,to understand its biological properties.METHODS：Density gradient centrifugation cultivation was utilized to culture rabbit BMSCs.The morphology and characteristics of cultured cells were observed.The surface markers CD29,CD34,CD44,CD45,CD166 and HLA-DR of cells were identified by flow cytometry,and its biological properties were identified.RESULTS AND CONCLUSION：High purity of rabbit BMSCs could be achieved by the density gradient centrifugation.The expressions of cell phenotypes were shown as follows respectively：CD29＋,CD34-,CD44＋,CD45-,CD166＋,HLA-DR-.Under appropriate conditions,BMSCs were induced to differentiate into osteoblasts and cartilage cells.