两种血细胞分离机干/祖细胞采集和移植效果的比较

背景：外周血造血干/祖细胞通过血细胞分离机进行体外收集,血细胞分离机的性能、工作状况将直接关系到采集物的特性和数量,从而直接影响受者的造血重建。目的：比较Fenwal CS-3000Plus与Amicus两种血细胞分离机采集外周血造血干/祖细胞及受者移植效果。方法：共入选接受异基因外周血干细胞移植患者51例,Fenwal CS-3000Plus组27例,平均年龄（34.2±10.6）岁;Amicus组24例,平均年龄（35.4±12.1）岁。比较两组采集物有核细胞数、CD34＋细胞数、采集效率、红细胞与血小板采集量及造血重建时间。结果与结论：两组采集物的有核细胞数、CD34＋细胞数、采集效率、红细胞采集量及造血重建时间差异均无显著性意义;Fenwal CS-3000Plus组血小板采集量明显高于Amicus组（P〈0.01）。结果显示Fenwal CS-3000Plus与Amicus血细胞分离机在分离外周血干/祖细胞方面和移植效果没有差异;血小板较低被采集者使用Amicus血细胞分离机采集可能更为安全。

Comparison of two blood cell separators in collection of peripheral blood stem cell/progenitor cells and effectiveness of transplantation

BACKGROUND：Peripheral blood stem cells/progenitor cells were collected in vitro using blood cell separators,which directly affects the features and quantity of collected cells,additionally,affects hematopoietic reconstitution of recipients.OBJECTIVE：To evaluate the efficiencies of Fenwal CS-3000 Plus and Amicus blood cell separators in collecting peripheral blood stem cells/progenitor cells and effectiveness of transplantation.METHODS：Totally 51 cases received allogenic peripheral blood stem cells transplantation were selected,including 27 cases,mean aged（34.2±10.6） years,who performed stem cell apheresis procedures using Fenwal CS-3000 Plus blood cell separator and 24 donors,mean aged（35.4±12.1） years,using Amicus blood cell separator.The number of nucleated cells and CD34＋ cells,collection efficiency,collection amount of red blood cell and the time of the hematopoieteic reconstitution of two devices were compared.RESULTS AND CONCLUSION：There were no significant differences in the total number of nucleated cells and CD34＋ cells,collection efficiency,collection amount of red blood cell and the time of the hematopoieteic reconstitution between the two devices.However,the amount of platelet in the stem/progenitor cell products of the Fenwal CS-3000Plus group was obviously greater than that of the Amicus group（P 0.01） .There is no difference in the collection efficiencies of the two blood cell separators in the peripheral blood stem cells/progenitor cell and the effectiveness of transplantation.The Amicus cell separator is better for the donors with thrombocytopenia.