护骨素基因T245G多态性与老年人骨密度关系的部位及性别差异水

背景：作为骨折发生的重要临床预测因子，骨密度在一定程度上由遗传因素决定。护骨素綦凶是骨质疏松症发病中的晕要候选摹闲。目的：探讨护骨索基因T245G多念性与骨密度的相关性。方法：选取2008-09／2010-04往北京大学人民医院进行常规查体的老年人281名，其中男182名，女99名。应用PCR-RFLP结合DNA测序榆测护骨素基因T245G多态件，使用-烈能X射线骨密度测量仪测定受试者腰椎、髋部标准位置及前臂的。旨密度。同时收集受试者的生化指标及临床观察项目。应用ANOVA方法分析护骨素基因T245G多态性与各检测指标的关系。结果与结论：在老年男性及绝经后女性中，T245G基因T，G等化基凶频率分布差异无显苫性意义（P〉0.05）。在老年男性中，GG和TG基凶型具仃较高的腰椎骨密度，而TT基吲型的腰椎骨密度较低（P〈0.05），Ward＇S三角区及前臂骨密度在各基因型问差异无显著件意义（P〉0.05）。北绝经后女性中，T245G多念性与骨密度无关，说明护骨索基凶与老年男性腰椎骨密度仃关。

Association between T245G polymorphisms in the osteoprotegerin gene and bone mineral density in elderly individuals

BACKGROUND： As a primary clinical predictor of fracture risk, bone mineral density （BMD） is partly genetically determined. Osteoprotegerin （OPG） is one important candidate gene in the pathogenesis of osteoporosis. OBJECTIVE： To investigate the association between T245G polymorphisms in the OPG gene and BMD. METHODS： A total of 281 elderly men and postmenopausal women, 182 males and 99 females, who received routine examinations at Peking University People＇s Hospital between September 2008 and April 2010 were included in this study. T245G polymorphisms in the OPG gene was detected by polymerase chain reaction-restriction fragment length polymorphism together with DNA sequencing. The BMD of the lumbar spine, Ward＇s triangle, and forearrm was determined by dual energy X-ray absorptiometry. Clinical variables and biochemical measurements were collected simultaneously. The association between T245G polymorphisms and each detection index was analyzed using analysis of variance. RESULTS AND CONCLUSION： The distribution of T245G genotype （alleles T, G） had no difference in elderly men or postmenopausal women （P〉0.05）. The GG genetype and TG genotype had higher lumbar spine BMD and TT genotype had lower lumbar spine BMD （P〈0.05）. There was no difference in BMD of the Ward＇s triangle or forearm among different genotypes （P〉0.05）. Association between T245G polymorphism and BMD was not found in postmenopausal women. These findings indicate that OPG gene is related to lumbar spine BMD in elderly men.