核因子kB、基质金属蛋白酶3在退变腰椎间盘组织中的表达

背景：对于椎间盘退变的原因一直是围内外学者研究的重点。近年来随着研究的深入．在椎间盘退变过程中多种细胞因子、黏附分子、趋化因子、炎症递质、蛋白质酶等发挥着重要作用。目的：比较正常腰椎间盘组织及退变腰椎间盘组织中核因子KB、基质金属蛋白酶3的表达，分析两者的相关性。方法：收集唐山市第二医院脊柱外科手术切除退变椎间盘组织68例（病变组），腰椎爆裂骨折正常椎间盘组织10例（对照组），应用苏木精-伊红染色、免疫组织化学方法和ELISA方法检测核因子KB、基质金属蛋白酶3的表达，并对两者相关性进行分析。结果与结论：苏木精-伊红染色结果显示对照组呵见纤维环和髓核结构，腰椎间盘髓核的软骨细胞表现为不规则的软骨陷窝，病变组细胞增殖明显，细胞核呈圆形或卵圆形，髓核细胞胞质呈空泡状。免疫组织化学方法显示病变组核因子kB、基质金属蛋白酶3吸光度值明显高于对照组（P〈0．05）。ELISA检测病变组核因子kB、基质金属蛋白酶3的表达均高于对照组（P〈0．05）。两者具有正相关性（r=0．6430，P〈0．01）。

Expression of nuclear factor kappa B and matrix metalloproteinases 3 in intervertebral disc degeneration

BACKGROUND： During intervertebral disc degeneration, various cytokines, adhesion molecules, chemokines, inflammatory neurotransmitters, protein enzymes play an important role. OBJECTIVE： To study the expression and significance of nuclear factor kappa B （NF-KB） and matrix metalloproteinases 3 （MMP-3） in normal disc tissue and intervertebral disc degeneration. METHODS： Sixty-eight patients with intervertebral disc degeneration from surgical intervention for cervical disc herniation （lesion group） and 10 cases of normal disc tissue （control group） were included. The expressions of NF-KB and MMP-3 were tested by hematoxylin-eosin staining, immunohistochemistry method and ELISA. RESULTS AND CONCLUSION： The NF-KB and MMP-3 positive expression rate was higher in the control group （P 〈 0.05）. There was a positive correlation （r=0.643 0, P 〈 0.01 ）. It indicated that NF-KB and MMP-3 may have important effects on intervertebral disc degeneration.