马钱子碱诱导人多发性骨髓瘤细胞株U266细胞凋亡与bax基因的表达

背景：马钱子碱具有抗肿瘤作用,而其对人多发性骨髓瘤细胞生长的影响尚不明确。目的：探讨马钱子碱诱导人多发性骨髓瘤U266细胞凋亡的机制。方法：取对数生长期的U266细胞,分别用0,0.05,0.1,0.2,0.4g/L的马钱子碱干预12,24,48h,通过MTT法测得IC50,并以此浓度干预U266细胞,通过形态学观察、RT-PCR法检测马钱子碱对人多发性骨髓瘤U266细胞株的诱导凋亡作用。结果与结论：马钱子碱诱导U266的凋亡呈时间和浓度依赖性（P〈0.05）,其作用48h的IC50为0.16g/L。在马钱子碱诱导的凋亡细胞中可见凋亡小体,同时,细胞中的促凋亡基因bax的表达量随马钱子碱作用时间的延长逐渐增加（P〈0.01）。说明0.4g/L浓度范围内的马钱子碱具有诱导U266细胞凋亡的作用,这种作用具有浓度和时间依赖性,可能是通过激活bax基因途径实现的。

Effects of brucine on apoptosis of multiple myeloma cell line U266 and bax gene expression

BACKGROUND：Brucine has anti-tumor effect.However,its influence on growth of multiple myeloma cells is not clear.OBJECTIVE：To investigate apoptotic mechanism of brucine on human multiple myeloma.METHODS：Logarithmic growth phase of U266 cells were treated with 0,0.05,0.1,0.2,and 0.4 g/L brucine for 12,24,and 48 hours,measured IC50 by MTT,and interfere the U266 cells.The apoptotic effect of brucine on human multiple myeloma cell line-U266 was observed by morphological observation and RT-PCR.RESULTS AND CONCLUSION：Brucine induced U266 apoptosis showed a time-and concentration-dependent manner （P〈0.05）.The IC50 level was 0.16 g/L at 48 hours after intervention.Apoptotic body could be found in brucine treated-apoptotic cells,meantime,the expression of pro-apoptosis gene bax was gradually increased with time prolonged （P〈0.01）.It is suggest that 0.4 g/L concentration brucine induced U266 apoptosis,and the effect showed in a concentration-and time-dependent manner,which possibly through the activation of bax pathway.