EBC中8-isoPG测定在不同肺部疾病中的临床意义

目的研究ARDS、OSAHS和AECOPD患者的EBC中8-isoPG浓度变化及临床意义。对象和方法选择江苏省南通大学第二附属医院的ARDS、OSAHS和AECOPD患者,收集ARDS患者入院第一天、OSAHS患者晨起、AECOPD患者以及健康对照组的EBC;采用ELISA法检测EBC中的8-isoPG浓度。结果 1.ARDS组、OSAHS组、AECOPD组和健康对照组EBC中8-isoPG浓度为(52.19±11.51 ng/L)、(14.93±1.39 ng/L)、(6.74±3.38 ng/L)、(3.21±0.97 ng/L)];2.ARDS、OSAHS、AECO-PD患者EBC中8-isoPG均高于健康对照组,三者EBC中8-isoPG浓度顺序排列为:ARDS组>OSAHS组>AECOPD组(P<0.01)。结论 EBC中8-isoPG可以反应多种肺部疾病的炎症及氧化应激程度。

Clinical significance of the detection of 8-isoPG in exhaled breath condensate of different lung diseases

Objective To investigate the clinical significance of the detection of 8-iso-prostaglandin （8-isoPG） in exhaled breath condensate of patients with acute respiratory distress syndrome （ARDS）, obstructive sleep apnea/hypopnea syndrome （OSAHS） and a- cute exacerbation of chronic obstructive pulmonary disease （AECOPD）. Methods The EBC was collected from the patients with ARDS, OSAHS, AECOPD and healthy controls, and the level of 8-isoPG was measured by enzyme linked immunosorbent assay. Results The concentrations of 8-isoPG in EBC of all patients and the healthy controls were 52. 19 ±11.51 ng/L （ ARDS）, 14. 93 ± 1.39 ng/L （ OS- AHS）, 6. 74± 3.38 ng/L （AECOPD）, and 3.21 ± 0. 97 ng/L （healthy controls）. The levels of 8-isoPG in all patients were higher than that in the healthy controls. Conclusion The level of 8-isoPG in EBC can reflect the inflammation of different lung diseases and the de- gree of oxidative stress.