细胞毒性T淋巴细胞相关抗原4/CT60基因多态性与溃疡性结肠炎的关系

目的探讨细胞毒性T淋巴细胞相关抗原4（CTLA4）CT00位点基因多态性与中国湖北地区汉族人溃疡性结肠炎（UC）的相关性。方法共收集64例UC患者和109例正常对照静脉血标本提取基因组DNA。采用多聚酶链反应．限制性片段长度多态性分析（PCR-RFLP）方法检测CT60G／A（rs3087243）位点的多态性，计算CTLA4的基因型和等位基因频率。结果CT60G／A基因型分布及等位基因频率，UC组与正常对照组比较差异无显著性，中国湖北地区汉族人群CTLA4CT60基因型和等位基因分布与日本人群相似。而与西班牙人群分布存在显著差异。结论CT60基因多态性与中国湖北汉族人UC易感性无关。

Association between the cytotoxic T lymphocyte associated antigen 4/CT60 gene polymorphism and ulcerative colitis in the Chinese

Objective To explore the relationship between the cytoxic T lymphocyte associated antigen 4/CT60 gene polymorphism and Chinese Han patients with UC.Methods Unrelated 64 Chinese Han patients with UC and 109 healthy controls were studied.The dimorphisms CT60 were genotyped using a polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP).The genotype and the allelic frequency were calculated.Results There were no significant differences in the distribution of CTLA4 CT60 genotype and allelic frequency between UC patients and controls. There were significant differences in the distribution of CTLA4 CT60 genotype and allelic frequency between Chinese and Spanish.While between Chinese and Japanese,there were not.Conclusion There was no relationship between CTLA4 CT60 gene polymorphisms and genetic susceptibility of Chinese Han UC patients.