| 桃红四物汤含药血清对过氧化氢损伤的人脐静脉内皮细胞的保护作用 |
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| 引用本文： | 刘竹青,尹登科,韩岚,李白坤,彭代银.桃红四物汤含药血清对过氧化氢损伤的人脐静脉内皮细胞的保护作用[J].中国中药杂志,2013,38(3):402-406. |
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| 作者姓名： | 刘竹青 尹登科 韩岚 李白坤 彭代银 |
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| 作者单位： | 安徽中医学院药学院现代中药安徽省重点实验室,安徽合肥,230031 |
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| 基金项目： | 国家自然科学基金项目(81073091);安徽省高校自然科学基金项目(KJ2011A189);安徽高校优秀人才基金项目(2010SQRL10) |
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| 摘 要： | 目的:研究桃红四物汤含药血清对过氧化氢损伤的人脐静脉内皮细胞(HUVECs)的保护作用.方法:SD大鼠灌胃给予桃红四物汤提取液(生药1.75 g·mL-1),每天2次,按10 mL·kg-1连续给药3d后,制备桃红四物汤含药血清.MTT法考察桃红四物汤含药血清(终浓度为5％,10％,15％)预处理对H2O2诱导细胞活力减少的作用；显微镜观察细胞形态学变化；以丙二醛(MDA)和乳酸脱氢酶(LDH)含量、超氧化物歧化酶(SOD)活性以及AO/EB染色考察桃红四物汤含药血清对HUVECs凋亡及抗氧化能力的影响；Western blot法检测细胞Caspase-3的表达.结果:与空白血清对照组比较,经400 μmol·L-1H2O2刺激后细胞活性显著降低,含药血清组可显著提高SOD活力、降低MDA和LDH含量,并抑制Caspase-3表达.AO/EB染色及相差倒置显微镜也观察到桃红四物汤含药血清能降低H2O2诱导的细胞凋亡.结论:桃红四物汤含药血清对H2O2诱导的HUVECs损伤有显著地保护作用.
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| 关 键 词： | 桃红四物汤 含药血清 人脐静脉内皮细胞 过氧化氢 |
| 收稿时间： | 2012/6/17 0:00:00 |
Protective effect of medicated serum prepared with Taohong Siwu Tang on hydrogen peroxide-induced human umbilical vein endothelial cells |
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| LIU Zhu-qing,YIN Deng-ke,HAN Lan,LI Bai-kun and PENG Dai-yin.Protective effect of medicated serum prepared with Taohong Siwu Tang on hydrogen peroxide-induced human umbilical vein endothelial cells[J].China Journal of Chinese Materia Medica,2013,38(3):402-406. |
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| Authors: | LIU Zhu-qing YIN Deng-ke HAN Lan LI Bai-kun and PENG Dai-yin |
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| Institution: | School of Pharmacy, Anhui University of Traditional Chinese Medicine, Anhui Key Laboratory of Modern Chinese Medicine, Heifei 230031, China;School of Pharmacy, Anhui University of Traditional Chinese Medicine, Anhui Key Laboratory of Modern Chinese Medicine, Heifei 230031, China;School of Pharmacy, Anhui University of Traditional Chinese Medicine, Anhui Key Laboratory of Modern Chinese Medicine, Heifei 230031, China;School of Pharmacy, Anhui University of Traditional Chinese Medicine, Anhui Key Laboratory of Modern Chinese Medicine, Heifei 230031, China;School of Pharmacy, Anhui University of Traditional Chinese Medicine, Anhui Key Laboratory of Modern Chinese Medicine, Heifei 230031, China |
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| Abstract: | |
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| Keywords: | Taohong Siwu Tang medicated serum human umbilical vein endothelial cell hydrogen peroxide |
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