超滤法测定牛蒡苷及牛蒡苷元的血浆蛋白结合率

目的:测定牛蒡苷及牛蒡苷元的血浆蛋白结合率.方法:采用超滤法结合高效液相色谱法对牛蒡苷及牛蒡苷元与大鼠血浆以及健康人血浆蛋白结合率进行测定.结果:牛蒡苷与大鼠血浆在64.29,32.14,16.07 mg·L-1-下的血浆蛋白结合率分别为(71.2±2.0)％,(73.4±0.61)％,(78.2±1.9)％,与健康人血浆的血浆蛋白结合率分别为(64.8±3.1)％,(64.5±2.5)％,(77.5±1.7)％;牛蒡苷元与大鼠血浆在77.42,38.71,19.36 mg·L-1下的血浆蛋白结合率分别为(96.7±0.41)％,(96.8±1.6)％,(97.3±0.46)％,与健康人血浆的血浆蛋白结合率分别为(94.7±3.1)％,(96.1±0.3)％,(97.9±1.3)％.结论:牛蒡苷与大鼠血浆蛋白有中等强度的结合,略高于与人血浆蛋白结合率;牛蒡苷元与大鼠血浆和健康人血浆均有很强的结合.

Determination of plasma protein binding rate of arctiin and arctigenin with ultrafiltration

Objective: To determine the plasma protein binding rate of arctiin and arctigenin. Method: The ultrafiltration combined with HPLC was employed to determine the plasma protein binding rate of arctiin and arctigenin as well as rat plasma and healthy human plasma proteins. Result: The plasma protein binding rate of arctiin with rat plasma at the concentrations of 64.29, 32.14, 16.07 mg·L^-1 were (71.2±2.0)%, (73.4±0.61)%, (78.2±1.9)%, respectively; while the plasma protein binding rate of arctiin with healthy human plasma at the above concentrations were (64.8±3.1)%, (64.5±2.5)%, (77.5±1.7)%, respectively. The plasma protein binding rate of arctigenin with rat plasma at the concentrations of 77.42, 38.71 , 19.36 mg·L^-1 were (96.7±0.41)%, (96.8±1.6)%, (97.3±0.46)%, respectively; while the plasma protein binding rate of arctigenin with normal human plasma at the above concentrations were (94.7±3.1)%, (96.8±1.6)%, (97.9±1.3)% , respectively. Conclusion: The binding rate of arctiin with rat plasma protein was moderate, which is slightly higher than the binding rate of arctiin with healthy human plasma protein. The plasma protein binding rates of arctigenin with both rat plasma and healthy human plasma are very high.