| 基于UPLC-TQ-MS考察不同树龄果用银杏叶萜内酯含量变化规律 |
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| 引用本文： | 姚鑫,周桂生,唐于平,钱叶飞,尚尔鑫,宿树兰,钱大玮,段金廒.基于UPLC-TQ-MS考察不同树龄果用银杏叶萜内酯含量变化规律[J].中国中药杂志,2013,38(3):376-380. |
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| 作者姓名： | 姚鑫 周桂生 唐于平 钱叶飞 尚尔鑫 宿树兰 钱大玮 段金廒 |
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| 作者单位： | 南京中医药大学江苏省方剂高技术研究重点实验室,江苏南京,210046 |
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| 基金项目： | 国家科技支撑计划项目(2011BAI04B03);国家教育部"新世纪优秀人才支持计划"项目(NCET-09-0163);江苏高校优势学科建设工程项目(ysxk-2010) |
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| 摘 要： | 目的:建立超高效液相色谱串联三重四级杆质谱法(UPLC-TQ-MS)同时测定银杏叶中4个萜内酯成分的含量.方法:采用ACQUITYTM UPLC BEH C18(2.1 mm×100mm,1.7μm)色谱柱,以水(A)-甲醇(B)为流动相,等度洗脱(70％A),流速0.4 mL·min-1,柱温30℃;Waters XevoTM TQ质谱仪以多反应离子监测(MRM)方式进行负离子检测.结果:各成分在5.0 min内分离良好,在相应的浓度范围内与峰面积呈良好的线性关系,相关系数均＞0.990 3;加样回收率(n=6)为98.83％～103.9％,RSD均小于3％.不同树龄果用银杏叶中萜内酯的含量差异较大,低树龄银杏叶含总内酯较高,随着树龄的增长,内酯含量逐渐降低.结论:该方法简便、快速、重复性好、专属性强,适用于银杏叶中萜内酯类成分的快速定量分析.
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| 关 键 词： | UPLC-TQ-MS 果用银杏叶 萜内酯 树龄 |
| 收稿时间： | 2012/10/13 0:00:00 |
Determination of terpene lactones in Ginkgo biloba leaves in different ages by UPLC-TQ-MS |
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| YAO Xin,ZHOU Gui-sheng,TANG Yu-ping,QIAN Ye-fei,SHANG Er-xin,SU Shu-lan,QIAN Da-wei and DUAN Jin-ao.Determination of terpene lactones in Ginkgo biloba leaves in different ages by UPLC-TQ-MS[J].China Journal of Chinese Materia Medica,2013,38(3):376-380. |
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| Authors: | YAO Xin ZHOU Gui-sheng TANG Yu-ping QIAN Ye-fei SHANG Er-xin SU Shu-lan QIAN Da-wei and DUAN Jin-ao |
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| Institution: | Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China |
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| Abstract: | |
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| Keywords: | UPLC-TQ-MS Ginkgo biloba leaves terpene lactones tree age |
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