| 循环高速逆流色谱从曼地亚红豆杉枝叶提取物中分离紫杉醇和三尖杉宁碱 |
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| 引用本文： | 苏静,谈锋,谢峻,冯巍,陈斌.循环高速逆流色谱从曼地亚红豆杉枝叶提取物中分离紫杉醇和三尖杉宁碱[J].中草药,2009,40(10):1569-1572. |
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| 作者姓名： | 苏静 谈锋 谢峻 冯巍 陈斌 |
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| 作者单位： | 西南大学生命科学学院,三峡库区生态环境教育部重点实验室,重庆市三峡库区植物生态与资源重点实验室,重庆400715 |
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| 基金项目： | 国家"863"课题资助项目 |
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| 摘 要： | 目的 研究曼地亚红豆杉枝叶提取物中分离紫杉醇及其类似物三尖杉宁碱的方法.提高紫杉醇的回收率及质量分数.方法 曼地亚红豆杉枝叶提取物先经Al2O3柱纯化后,再以正己烷-醋酸乙酯-甲醇-乙醇-水(5:7:5:1:6.5)为溶剂系统,利用循环高速逆流色谱分离紫杉醇和三尖杉宁碱.结果 经循环高速逆流色谱循环一次后,三尖杉宁碱与紫杉醇即达到基线分离,紫杉醇质量分数达98.2%以上,回收率83.0%.结论 循环HSCCC法为分离紫杉醇和三尖杉宁碱提供了一条简便、快速的方法,使制备高质量分数、高回收率的紫杉醇成为可能.
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| 关 键 词： | 曼地亚红豆杉枝叶 紫杉醇 三尖杉宁碱 循环高速逆流色谱 |
| 修稿时间： | 2009/1/27 0:00:00 |
Separation of paclitaxel and cephalomanine from extracts in Taxus media branches and leaves by recycling high-speed counter-current chromatography |
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| SU Jing,TAN Feng,XIE Jun,FENG Wei and CHEN Bin.Separation of paclitaxel and cephalomanine from extracts in Taxus media branches and leaves by recycling high-speed counter-current chromatography[J].Chinese Traditional and Herbal Drugs,2009,40(10):1569-1572. |
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| Authors: | SU Jing TAN Feng XIE Jun FENG Wei and CHEN Bin |
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| Institution: | Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Key Laboratoryof Plant Ecology and Resources in Three Gorges Reservoir Region, School of Life Science, Southwest China University, Chongqing 400715, China;Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Key Laboratoryof Plant Ecology and Resources in Three Gorges Reservoir Region, School of Life Science, Southwest China University, Chongqing 400715, China;Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Key Laboratoryof Plant Ecology and Resources in Three Gorges Reservoir Region, School of Life Science, Southwest China University, Chongqing 400715, China;Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Key Laboratoryof Plant Ecology and Resources in Three Gorges Reservoir Region, School of Life Science, Southwest China University, Chongqing 400715, China;Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Key Laboratoryof Plant Ecology and Resources in Three Gorges Reservoir Region, School of Life Science, Southwest China University, Chongqing 400715, China |
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| Abstract: | study the met hod for separating paclitaxel from the extract sin Taxus me diabranches and leaves and cephalo manine,and to increase reco very rate and content of paclitaxel.Methods The extract in T1me diabranches and leaves was purified by firstly. |
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| Keywords: | branches and leaves of T a x us medi a cv1 Hicksii paclitaxel cep halo manine recycling higH2 speed co unter2 current chro mato grap hy |
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