肝再生磷酸酶、早期生长反应因子-1与胃癌侵袭转移的相关性

目的研究肝再生磷酸酶（PRLs）、早期生长反应因子-1（Egr-1）在胃癌组织中的表达情况及其临床意义。方法采用RT-PCR方法检测胃癌组织、癌旁及正常胃黏膜中PRLs、Egr-1 mRNA的表达水平。采用Western blot方法检测胃癌组织、癌旁及正常胃黏膜中PRLs、Egr-1蛋白的表达水平。结果PRLs和Egr-1在基因水平及蛋白水平均可在部分胃癌组织、癌旁、正常胃黏膜中检测到;在基因水平及蛋白水平的检测均发现,PRLs和Egr-1在胃癌组织中表达最高,癌旁次之,在正常组织表达最弱（P〈0.05）;Egr-1蛋白的阳性表达率与胃癌淋巴结转移、肿瘤TNM分期相关。结论PRLs和Egr-1的表达与胃癌的发生发展相关,Egr-1蛋白的检测对胃癌预后判定有一定意义。

Study on the Invasion and Metastasis Impact of Phosphatases in Regenerating Liver and Early Growth Response Factor in Gastric Cancer

Objective To study the expression and clinical significance of phosphatases in regenerating liver（PRLs） and early growth response factor-1 （Egr-1） in gastric cancer. Method The expression of PRLs and Egr-1 mRNA was examined by RT-PCR, and the expression of PRLs and Egr-1 protein was examined by western blot. Results PRLs mRNA, PRLs protein, Egr-1 mRNA and Egr-1 protein could be detected in some of cases, and they expressed higher in gastric carcinoma specimens than in para-tu- morous tissues and expressed the lowest in normal gastric mucosa（ P 〈 0.05 ）. The positive expression rate of Egr-1 protein had a close correlation with lymph node metastasis and TNM stages in gastric cancer. Conclusion The high expression level of PRLs and Egr-1 in gastric cancer tissues implys that they may have a significant impact on the occurrence and development of gastric cancer. The expression of Egr-1 protein has an impact on the prognosis gastric cancer.