99^Tc^m-MIBI显像评价人肺腺癌荷瘤裸鼠多药耐药状态

目的采用99^Tc^m-甲氧基异丁基异腈（MIBI）SPECT显像技术在BALB/c裸鼠的SPCA-1人肺腺癌移植瘤模型上观察应用维拉帕米逆转前后的肿瘤细胞摄取显像剂的情况,以探讨应用该方法评价肿瘤多药耐药状态的可行性。方法荷瘤裸鼠随机分为维拉帕米逆转组和对照组,在应用维拉帕米逆转前后分别进行99^Tc^m-MIBISPECT显像及感兴趣区（ROI）半定量分析,观察肿瘤多药耐药状态变化情况,并将其结果与流式细胞仪检测结果进行对比。结果对照组与逆转组逆转后显像、逆转组逆转前显像与逆转后显像之间15、60、120min的肿瘤摄取比值（TUR）差异有高度统计学意义（P〈0.01）。对照组和逆转组滞留率（RI）均与流式细胞仪检测的P-gp蛋白表达阳性细胞的百分率成负相关（P〈0.05）。结论99^Tc^m-MIBI显像与SPCA-1人肺腺癌肿瘤细胞P-gp的表达成负相关,可以较好地显示肿瘤的多药耐药状态,同时也可动态监测维拉帕米对SPCA-1人肺腺癌多药耐药逆转的效果。

Experimental Research of ~(99)Tc~m-MIBI SPECT in Detecting Multidrug Resistance Status on Tumor Nude Mice

Objective To analyze 99^Tc^m-MIBI images separately before and after VPL in nude mice bearing human lung tumors in order to find a feasible way in evaluating MDR status of tumor with 99^Tc^m-MIBI image. Methods Analysis of 99^Tc^m-MIBI images was performed at 15 min,60 min and 120 min after injecting 99^Tc^m-MIBI 7.4 MBq in female BALB/c nude mice bearing human lung tumors. The tumor uptake rate（TUR） and retention rate（RI） were calculated and the data were analyzed. P-gp albumen ex-pression was determined by flow cytometry. Results Significance difference in TUR was observed for at the 15th,60th or 120th min between the imaging in the control group versus in the second imaging in VPL reverse group as well as between the first imaging and the second imaging in the reverse group. Furthermore,there was negative correlation between retention rate （RI） and P-gp albumen expression. Conclusion These results for the first time demonstrate that the results of 99^Tc^m-MIBI imaging exhibits a negative correlation to P-gp albumen expression,indicating that analysis of 99^Tc^-MIBI imaging may be a potential indicator for MDR status of tumor and can be used to monitoring the role of VPL in revertingthe multidrug resistance.