人类辅助生殖技术中DAY3胚胎质量与囊胚形成相关性分析

目的探讨人类辅助生殖技术中,day3胚胎质量与囊胚形成的相关性。方法将符合纳入标准的130例患者day3胚胎移植后剩余胚胎进行囊胚培养至day6,分析患者年龄、day3胚胎分级、day3胚胎细胞数及培养时间与囊胚形成的关系。结果患者年龄与囊胚形成无明显相关;囊胚形成率与day3胚胎质量评分和细胞数呈显著正相关,day3评分为Ⅰ-Ⅱ级胚胎囊胚形成率明显高于Ⅲ-Ⅳ级胚胎,day3细胞数≥6的胚胎囊胚形成率明显高于≤5的胚胎囊胚形成率。综合分析day3胚胎分级、细胞数、培养时间与囊胚形成率的关系,囊胚形成率最高的是≥6细胞Ⅰ-Ⅱ级胚胎,其次是≥6细胞Ⅲ-Ⅳ级胚胎,≤5细胞的其他两组囊胚形成很低,各组胚胎day6囊胚形成率均明显高于day5。结论 day3胚胎的细胞数、质量分级及囊胚的培养天数都能够影响囊胚的形成,其中day3胚胎的细胞数更为重要,培养至day6使day3胚胎质量较差和发育迟缓的胚胎有机会形成囊胚,有效地筛选和保存了有一定发育潜能的胚胎。

Correlation analysis of the quality of the 3th embryo with the blastocyst formation during human ART

Objective: To analyze the correlation of the quality of the 3th embryo with the blastocyst formation during human ART.Methods: In this study,All 130 patients accorded with our study standard.The 3th surplus embryos after transferred were cultured to the 6th days for the blastocyst formation.We tried to find the relationship among the patient′s age,the 3th day embryo quality score and cell number with blastocyst formation.Result: The patient′s age was not related with blastocyst formation.The remarkable result was the positive correlation beween blastulation rate with the day 3 embryo cell number or with the day3 embryo quality score.The blastocyst formation rate of the good quality embryo(Ⅰ-Ⅱ)was significantly higher than that of the poor quality embryo(Ⅲ-Ⅳ).The blastocyst formation rate of the ≥6 cells embryos in the 3th days was higher than that of the ≤5 cells embryos.By comprehensive analysis about the correlation of the blastocyst formation with day3 embryo cell numbe,the day3 embryo quality score and culture time,the result showed that the≥6 cells Ⅰ-Ⅱdegree embryos had the highest blastocyst formation rate,the blastocyst formation rate of the≥6 cellsⅢ-Ⅳ degree embryos was higher.The blastocyst formation rate of the ≤5 cells embryos was lower than the ≥6 cells embryos.In all four groups embryos,the blastocyst formation rates in the 6th day were higher than in the 5th day.Conclusion: The day3 embryo cell number,quality score and culture time were important factors for the blastocyst formation,in which the 3th day embryo cell number was the most important.The culture to the 6th day brought some chance to blastocyst for surplus embryos which quality score was lower or developed slower.This culture progress effectually selected and saved the embryos which had potentiality to form the bastula.