非酒精性脂肪肝病患者血清脂联素水平及其在肝脏组织中的表达情况的研究

目的探讨非酒精性脂肪肝病(NAFLD)患者血清脂联素水平变化及其在肝脏组织中的表达情况,探讨脂联素在NAFLD发生、发展中的作用。方法选取NAFLD患者30例(NAFLD组)及非NAFLD的其他疾病患者30例(对照组)。计算两组患者的体质指数(BMI),比较两组患者血清空腹血糖(FBG)、空腹胰岛素(FINS)、总胆固醇(TC)、三酰甘油(TG)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、γ-谷氨酰转肽酶(GGT)、脂联素水平及胰岛素抵抗指数(HOMA-IR)。并用免疫组化方法检测脂联素在两组患者肝脏组织中的表达情况。结果NAFLD组患者BMI、ALT、GGT、TG、FINS水平及HOMA-IR均显著高于对照组,而脂联素水平则显著低于对照组,差异均有统计学意义(P<0.05);两组受试者血清AST、FBG、TC比较,差异无统计学意义(P>0.05)。NAFLD组患者肝活检标本中脂联素的平均光密度值显著低于对照组,差异有统计学意义(P<0.05)。结论脂联素在NAFLD的发生、发展中起重要作用。

The Study of Changes of Serum Adiponectin Level and the Expression of Adiponectin in Patients with Nonalcoholic Fatty Liver Disease

Objective To explore the role of adiponectin in the development and progression of nonalcoholic fatty liver disease(NAFLD) through detecting changes of serum adiponectin level and the expression of adiponectin in NAFLD.Methods 30 NAFLD patients and 30 patients with other diseases were included in the study.Body mass index(BMI),fasting blood glucose(FBG),fasting insulin(FINS),total cholesterol(TC),triglyceride(TG),alanine aminotransferase(ALT),aspartate aminotransferase(AST),gamma glutamyl transpeptidase(GGT),adiponectin and HOMA-IR(homeostasis model assessment insulin resistance) were measured.The expression of adiponectin in NAFLD was examined in the two groups using immunohistochemistry.Results The levels of BMI,ALT,GGT,TG,FINS and HOMA-IR were significantly higher in NAFLD group than in control group(all P<0.05).The levels of adiponectin were significantly lower in NAFLD group than in control group(all P<0.05).No statistically significant differences were found in the levels of AST,FBG and TC between NAFLD group and control group(all P>0.05).The average optical in adiponectin of the biopsy specimen in NAFLD group was significantly lower than that in control group(P<0.05).Conclusion Adiponectin plays a key role in the occurrence and development of NAFLD.