应用荧光图像对丹磺酰氯染色后角质层转化时间的评价

目的丹磺酰氯皮肤染色后,应用荧光图像及其灰度值对角质层的转化时间进行评价。方法 30名受试者的前臂内侧应用丹磺酰氯染色,染色后每天在伍德氏灯下肉眼评价染色强度,连续28d。同时每周应用相机摄取荧光图像2次,根据这些图像的染色强度得到荧光图像的肉眼评分。应用Photoshop软件分析摄取图像得到荧光图像的灰度值。结果经过28d的试验,皮肤染色逐渐脱落,而荧光图像的灰度值也随时间逐渐减小。荧光图像灰度值与伍德氏灯下肉眼评分、图像的肉眼评分具有较好的一致性。结论应用荧光图像评分及其灰度值可以客观地评价丹磺酰氯皮肤染色的脱落情况,提高试验评分的敏感性,并可获取数字化的结果。

The Evaluation of Stratum Corneum Turnover Time After Dansyl Chloride Staining by Fluorescence Image

Objective To evaluate stratum corneum turnover time after the dansyl chloride staining by fluorescence images and its grey values.Methods Thirty subjects were stained by dansyl chloride at inner forearm.The dyeing was evaluated by visual scoring under Wood’s lamp once a day in consistant 28 days.At the same time ultraviolet fluorescence images were taken by a fluorescence camera twice a week.Then we got the visual score of images base on the staining intensity of these pictures.The grey value of the fluorescence images were analyzed by Photoshop software.Results During 28 days,the dyeing at the study site disappeared gradually.And the grey value of fluorescence images decreased gradually as well.There were good correlation among the grey value of fluorescence image and the score of visual assessment under Wood’s lamp and the scores of image assessment.Conclusion The fluorescence images and its grey value could be the objective evaluation method for the stratum corneum turnover time by dansyl chloride staining.The sensitivity of evaluation could be improved and the fluorescence result could be got.