NDRG2基因在原发性肝细胞肝癌及正常肝组织中的表达研究

目的探讨NDRG2基因在人原发性肝细胞肝癌及正常肝组织中的表达及其临床意义。方法收集原发性肝细胞肝癌及正常肝组织标本,采用免疫组化ABC法、Western blot法及Real-time PCR法检测原发性肝细胞肝癌及正常肝组织标本中NDRG2蛋白和mRNA的表达情况,并结合图像分析,比较两者表达的差异。结果 NDRG2蛋白在肝癌组织及正常肝组织中的表达阳性率分别为16.67%(5/30)和100%(30/30),2组差异有统计学意义(P<0.05),且阳性表达呈现在细胞质中,细胞核中未见有表达。图像分析结果显示,NDRG2蛋白在肝癌组织中表达的相对含量为0.029 0±0.005 9,在正常肝组织中为0.109 2±0.002 8,前者低于后者,其差异具有统计学意义(P<0.05)。Western blot法检测结果与免疫组化染色结果相一致,在肝癌组织及正常肝组织中均检测到NDRG2蛋白,肝癌组织中NDRG2蛋白相对表达量为1.13±0.15,低于正常肝组织的1.57±0.18(P<0.05)。Real-time PCR分析结果显示肝癌组织中NDRG2 mRNA表达量为0.89±0.15,低于正常肝组织的1.48±0.17(P<0.05),但肝癌Edmondson-Steiner分级的各级肝癌组织之间NDRG2 mRNA表达量差异均无统计学意义(P>0.05)。结论 NDRG2基因在原发性肝细胞肝癌及正常肝组织中可能存在差异性表达,提示该基因可能参与了肝细胞肝癌的发生和发展,但其具体作用及调控机理有待进一步研究。

The Expression and Significance of NDRG2 Gene in Primary Hepatocellular Carcinoma and Normal Hepatic Tissues

Objective To explore the expression and function of NDRG2 gene in human primary hepatocellular carcinoma and normal hepatic tissues.Methods The immunohistochemical ABC method,Western blot,and Realtime PCR were used to investigate the expression and content of NDRG2 in human hepatocellular carcinoma and hepatic normal biopsies.Results The NDRG2 protein located in cytoplasm.The positive rate was 16.67%(5/30)and 100%(30/30)in hepatocellular carcinoma and normal hepatic tissues,respectively.The relative content of NDRG2 protein in hepatocellular carcinoma and normal hepatic tissues were 0.029 0±0.005 9 and 0.109 2±0.002 8.There were significant differences between human hepatocellular carcinoma and hepatic normal biopsies both in staining positive rates and relative content(P<0.05).The Western blot also agreed with the result,the expression level of NDRG2 protein in hepatocellular carcinoma and normal hepatic tissues was 1.13±0.15 and 1.57±0.18,respectively,there was significantdifference(P<0.05).Also,compared with normal hepatic tissues,the expression level of NDRG2 mRNA in carcinoma tissues was reduced significantly(0.89±0.15 vs.1.48±0.17,P<0.05).However,there were no significant differences in NDRG2 mRNA expression between Edmondson-Steiner grades.Conclusions There possibly have difference in NDRG2 expression between human primary hepatocellular carcinoma and normal hepatic tissue.NDRG2 gene may take part in the pathogenesis of human primary hepatocellular carcinoma.Futher study will be needed to study its mechanism and function.