流式细胞术检测肺癌患者外周血微转移的探讨

目的:应用流式细胞术检测肺癌患者外周血中的微转移。方法:外周血经F icoll梯度离心分离单核细胞后,用粘蛋白1(Muc in1,MUC1)单克隆抗体染色,应用流式细胞仪检测。结果:检测了96例肺癌患者外周血,发现29例患者外周血中有微转移。32例肺良性病变患者有1例患者外周血中有微转移。肺癌患者阳性率与患者病理分期、分化程度和细胞组织学类型密切相关(P<0.05)。结论:应用流式细胞术检测非小细胞肺癌患者外周血中的微转移,有助于判断肺癌转移倾向和预后。

Detecting micromatastasis in the peripheral blood of patients with lung cancer by flow cytometry

Objective:To detect micrometastasis in the peripheral blood of non small cell lung cancer(NSCLC) patients by flow cytometry(FCM).Methods: The monotype fraction in peripheral blood was isolated by Ficoll-Hypaque gradient centrifugation.The cells obtained were labeled with antibodies against MUC1,then analyzed by FCM.Results: Twenty nine cases out of 96 patients with NSCLC were found to have micromatastasis in the peripheral blood.One case was found to have micromatastasis in 32 lung benign patients.There was distinct correlation between detective rate of micromatastasis and pathological classification,cell differentiation of the cancer and P-TNM stage of the disease.(P<0.05).Conclusion: Examination of micromatastasis in the peripheral blood by FCM may be valuable in predicting metastasis and prognosis of NSCLC.