出生前氚水照射对仔代大鼠生长发育及神经行为的影响

利用多项指标研究出生前氚水照射对仔代大鼠生长发育及神经行为的影响。方法妊娠１３天的成年Ｗｉｓｔａｒ大鼠腹腔单次分别注入不同活度氚水，各组仔鼠氚累积吸收剂量分别为０，０．０４４，０．０８８和０．２６４Ｇｙ。观察氚β射线对仔鼠出生后生长发育及神经行为的影响。结果在０．０４４Ｇｙ组即可使仔鼠连续通道转弯数减少，Ｙ迷宫刺激回避反射逃避时间延长；在０．０８８Ｇｙ组即可使出牙发育延迟，平面翻正，负趋地性及听觉惊愕等新生反射和感觉功能出现的天龄延迟，Ｙ迷宫刺激单向回避反射无误率降低，条件反射达标率降低；０．２６４Ｇｙ组张耳、开眼发育延迟，前肢悬挂时间缩短，Ｙ迷宫刺激回避反射阳性率降低；绝大部分指标表现与剂量相关。结论出生前一定剂量的氚水照射可导致仔代大鼠生长发育的延缓及学习记忆功能的降低等神经行为的异常。

Effect of prenatal exposure to low dose beta radiation from tritiated water on postnatal growth and neuro behavior of rats.

Objective Effects of prenatal exposure to HTO(tritiated water) on postnatal growth and neurobehavior of rats were studied by determination of multiple parameters.Methods Pregnant adult Wistar rats were randomly assigned to 4 groups, of which 3 groups were irradiated with beta rays from tritiated water (HTO) by one single intraperitoneal injection on the 13th day of gestation. Offspring of these rats received cumulative doses of 0.000, 0.044, 0.088 and 0.264 Gy in utero, respectively, and were observed for the appearance of three physiologic markers (eye opening, pinna detachment, incisor eruption), the age of acquisition of two reflexes (surface righting, negative geotaxis) and sensuous function (auditory startle), movement and coordination functions and activity (forelimb hanging, continuous corridor activity), and learning and memory (electric avoidance reflex in Y maze, conditional reflex).Results Results for most parameters in the 0.044 and 0.088 Gy groups were different significantly from those in the controls and for most parameters a dose dependent effect was found. Conclusion Offspring of rats having received prenatal low dose irradiation from HTO showed delayed growth and abnormal neurobehavior.