CMV-PP65抗原检测在预防肾移植术后巨细胞病毒感染的临床价值

目的 探讨巨细胞病毒PP65抗原（CMV-PP65）检测在预防肾移植术后巨细胞病毒（CMV）感染的临床价值.方法 采用间接免疫荧光法和酶联免疫捕获法检测52例肾移植受者术后CMV-PP65抗原和CMV-IgM抗体.结果 52例肾移植受者中CMV-PP65抗原检测阳性25例,CMV-IgM抗体检测阳性17例,随访3~6个月,共15例发生CMV病,CMV-PP65抗原检测均阳性,CMV-PP65抗原检测的敏感度及阴性预测值均高于CMV-IgM抗体检测;25例患者CMV-PP65抗原检测术后首次出现阳性的平均时间为4.2±3.3周,17例患者CMV-IgM抗体检测术后首次出现阳性的平均时间为8.7±5.8周,两者比较有显著差异性（P〈0.05）.结论 CMV-PP65抗原检测可早期排除和预防肾移植术后CMV感染,值得临床推广应用.

The Clinical Value of CMV-PP65 Antigen Detection in the Prevention ofCytomegalovirus Infection After Renal Transplantation

ObjectiveTo investigate the clinical value of CMV-PP65 antigen detection in the prevention of cytomegalovirus infection after renal transplantation.MethodsThe CMV-PP65 antigen and CMV-IgM antibody of 52 cases with renal transplantation recipients were detected with the method of indirect immunofluorescence and enzyme-linked immune capture.ResultsAmong the 52 cases of kidney transplant,25 cases were tested positive with CMV-PP65 antigen,17 cases were tested positive with CMV-IgM antibody.All patients were followed up for 3~6 months,CMV occurred in 15 cases,and their CMV-PP65 antigen were detected positive,the detection sensitivity and negative predictive value of CMV-PP65 antigen detection were higher than that of CMV-IgM antibody detection.The average length for the first postsurgical positive detection of CMV-PP65 antigen with 25 cases was 4.2±3.3 weeks,while that of the CMV-IgM antibody with 17 cases was 8.7±5.8 weeks,with a significant difference between them (P<0.05).ConclusionCMV-PP65 antigen detection could be employed for the early elimination and prevention of CMV infection after renal transplantation.It was worthy of clinical popularization and application.