CDH1基因-160C/A基因多态性与鼻咽癌发病风险的研究

目的 本研究探讨CDH1基因-160C/A多态性在湖南汉族鼻咽癌人群中的分布及其与汉族鼻咽癌发病风险的相关性.方法 应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对280例湖南籍汉族鼻咽癌患者和291例湖南籍汉族非肿瘤对照组进行CDH1基因-160C/A SNP检测.比较基因型分布和发病风险的关系.结果 CDH1基因-160C/A多态的CC、CA、AA基因型在病例组中的分布频率分别为156（55.7%）,103（36.8%）,21（7.5%）;在对照组的分布频率分别为178（61.2%）,102（35.0%）,11（3.8%）;（χ2=0.597,P=0.440）,符合Hardy-Weinberg平衡（P＞0.05）;两组间分布的差异无统计学意义（P〉0.05）.AA基因型显著性地提高鼻咽癌的发病危险（OR=2.96;95%CI：1.12~4.81）;携带A等位基因与鼻咽癌患者发生淋巴结转移有关联性（OR=3.07;95%CI：1.88~5.02）.结论 CDH1基因-160C/A多态性可能与汉族鼻咽癌发病的遗传易感性及鼻咽癌患者发生淋巴结转移密切相关.

The Susceptibility of Nasopharyngeal Cancer andCDH1 Gene-160-C/A Gene Polymorphism

Objective This study investigated the CDH1 gene-160C/A polymorphism in Hunan province,China Han population distribution of nasopharyngeal carcinoma and its relationship with Han Chinese in relevance of the risk of naso- pharyngeal carcinoma incidence. Methods Application of polymerase chain reaction-restriction fragment length polymor- phism（PCR-RFLP） of the 280 cases of nasopharyngeal carcinoma patients and 291 cases of Han non-cancerous group CDH1 gene-160C/A SNP detection. Comparison of genotype distribution and the relationship between the incidence of risk ;risk OR and 95% CI application of non-conditional Logistic regression analysis and calculation. Results CDH1 gene-160C/A polymorphism of the CC, CA, AA genotype in case group in the distribution of frequencies of 156（55.7% ）. 103 （36.8%）, 21 （7.5 % ） ; the distribution of frequencies in the control group were 178 （ 61.2% ）, 102 （ 35.0% ）, 11 （ 3.8% ） ; （ X^2 = 0. 597, P = 0. 440）, consistent with Hardy-Weinberg equilibrium（P 〉 0.05 ） ;the distribution between the two groups no sta- tistically significant difference （P 〉 0.05 ）. AA genotype significantly increase the incidence of nasopharyngeal cancer risk （OR = 2.96 ;95% CI：I. 12 -4.81 ） ;carrying A allele was associated with lymph metastasis in patients with nasopharyngeal carcinoma correlation（ OR = 3.07195% CI ： 1.88 - 5.02）. Conclusion CDH1 gene-160C/A polymorphism may be related to the pathogenesis of nasopharyngeal carcinoma in Han nationality and the genetic susceptibility to nasopharyngeal carcinoma in patients with lymph node metastasis.