| 用DNA探针技术鉴定鼠疫菌的研究 |
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| 引用本文： | 艾泽孜. 用DNA探针技术鉴定鼠疫菌的研究[J]. 地方病通报, 1997, 12(1): 33-34 |
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| 作者姓名： | 艾泽孜 |
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| 作者单位： | 新疆地方病防治研究所!乌鲁木齐市，830002 |
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| 摘 要： | 本试验采用的鼠疫菌ｆｒａ基因经ＰＣＲ扩增地高辛标记方法制备基因探针，通过菌落原位杂交试验进行部分鼠疫菌、假结核菌和大肠杆菌的鉴定、被试菌中ＥＶ、ＥＶ７６ｐａｒｉｓ、６１、Ｍ２３四株鼠疫菌呈现阳性反应，假结核菌和大肠菌均为阴性。
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| 关 键 词： | 鼠疫杆菌 fra基因 DNA探针 原位杂交 |
Study on Identifying Yersinia pestis with DNA Probe |
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| Abstract: | Y. pestis, Y. pseudotuberculosis and E.coli were identified by using the DNA probe of Y. pestis with PCR amplifications and labelled with DlG. The positive reaction occurred on EV, EV76Paris, 61, M23 of Y. Pestis and the negative reaction occurred on P1, P2, P3, S- l of Y. pseudotuberulosis and pBT238,40,32,23 of E. coli. The test indicated specificity of DNA probe fragen of Y. Pestis was high, but using the test method needs further study in plague control because the test examined the small amount bacteria. |
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| Keywords: | Y. pestis Probe fra gen DNA probe Hybridism in situ |
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