VEGF-C和VEGFR-3在非小细胞肺癌癌组织及癌旁组织中的表达及意义

目的通过对非小细胞肺癌患者肺癌组织中VEGF-C水平的表达以及对癌旁组织中淋巴管密度的测定来探讨VEGF-C和VEGFR-3在NSCLC肿瘤淋巴转移的意义。方法对55例无手术禁忌的NSCLC患者施行肺癌根治术,获得肺癌肿瘤组织,癌旁组织以及远离肿瘤的正常组织,应用VEGF-C抗体SP免疫组织化学等技术染色观察VEGF-C在细胞浆中的表达以及应用VEGFR-3抗体SP法标法淋巴管计数癌周组织中的微淋巴管密度（LMVD）。结果 （1）55例NSCLC患者癌组织中,40例VEGF-C呈阳性表达（占72.72%）,对照组正常组织中VEGF-C阳性仅5例（阳性率9.56%）,两者有显著差异（P〈0.005）。（2）55例癌旁组织中LMVD值为21.20±7.22个,而正常组织中LMVD值为9.48±5.52个,两者有显著差异（P〈0.01）。（3）试验组有肿瘤淋巴结转移者共计35例,其癌组织中VEGF-C阳性者为32例,3例为阴性;无肿瘤淋巴结转移者共计20例,12例阴性,8例阳性表达,两者有显著差异（P〈0.005）。（4）肺癌淋巴结转移者35例中,癌旁组织中LMVD值为27.42±6.38,比没有肺癌淋巴结转移者LMVD15.10±2.72亦显著增高（P〈0.05）。结论非小细胞肺癌组织中VEGF-C的高表达与癌旁组织淋巴管的高表达有密切关系,VEGFR-3的阳性脉管数与肺癌淋巴结转移相关联,可作为肺癌诱导生成淋巴管研究的量化指标。肿瘤组织的VEGF-C表达水平和对癌周组织淋巴管密度测定有助于对肺癌患者预后的判断和治疗方案的评价。

Expression and significance of VEGF-C and VEGFR-3 in non-small cell lung carcinoma

Objective To investigate the relationship and significance of VEGF-C expression in the serum lymph and in the lung tissue of non-small all lung carcinoma,and to investigate the role of VEGF-C in the lymph metastasis of non-small cell cancer.Methods S-p immunohistochemical staining was used to detect the expression of VEGF-C and its receptor VEGFR-3 of neoplasm tissue and peri-tu- moral tissue in 55 cases with non-small lung carcinoma.Results（1）Positive immunostainning in lymph node metastasis was observed in 35 of 55（63.64%）,positive immunostainning in lymph node no-metastasis was observed in 20 of 55 cases（36.36%）,there were signifi- cant difference（P〈0.05）.（2）The LMVD in lymph node metastasis was 26.57±7.23.The LMVD in lymph node no-metastasis was 13.53±8.55,there were significant difference（P〈0.01）.Conclusion There is a positive correlation between VEGF-c and VEGFR-3 over-expression in non-small cell lung cancer.The number of VEGFR-3 positive vessels is related to lymph node metastasis,detecting the VEGF-C in lymph is a new method to evaluate the detecting lymph-node metastasis and the effective of therapy.It would play an important role in future.