牙髓成牙本质细胞层毛细淋巴管的透射电镜研究

目的研究牙髓成牙本质细胞层中有无毛细淋巴管及其超微结构特点。方法取牙根发育完成的健康恒前磨牙15颗,纵向劈开后在2．5％戊二醛中4℃固定1h,完整地取出牙髓,切成截面积约为1mm^2,长约3mm的小长条,再在同一固定液中固定2h,后固定,包埋,半薄切片定位,选取5例牙髓进一步作超薄切片,铀铅染色,用透射电镜观察。结果在成牙本质细胞层中观察到具有典型毛细淋巴管超微结构特点的结构。结论在牙根发育完成的年轻恒前磨牙成牙本质细胞层中有毛细淋巴管,其具有与其他组织毛细淋巴管基本相似的超微结构特征。

Studies on the Lymphatic Capiliaries in the Odontoblast Layer of Pulp with Transmission Electron Microscope

Objective To study the lymphatic capiliaries in the odontoblast layer of pulp. Methods 15 healthy premolars were employed in the experiments whose roots had developed. They were cleaved longitudinally as soon as they were extracted and put into 2. 5% glutaraldehyde soulution for 1 hour at 4C. The pulps were taken out and cut into little pieces and re-immersed in the same soulution for 2 hours. Then they were postfixed, dehydrated and embedded. And semithin sections were made. 5 pulps were chosen to make ultrathin sections and observed by transmission electronmicroscope (TEM). Results In the odontoblast layer, structures which had typical characters of lymphatic capiliaries were found with TEM. Conclusion There are lymphtic capillaries in the odontoblast layer of pulps which are young and healthy. The characters of lymphtic capiliariy are similar to those of other tissues.