乙酸纤维膜在印迹细胞法免疫检测干眼病的应用

目的；研究乙酸纤维膜（cellulose acetate membrane,CAM)在眼球结膜印迹细胞检查（Conjunctival impression cytology)联合免疫组织化学染色的应用价值。方法：对门诊诊断为干眼病的24例病人，采用乙酸纤维膜、印迹细胞法获取干眼病人球结膜上皮细胞，用抗体TGF－β1进行免疫组织化学染色，光镜观察。结果：显微镜观察见乙酸纤维膜透明，TGF－β1染色阳性的球结膜上皮细胞胞膜清晰，胞浆呈棕色，胞核呈兰色。结论：采用乙酸纤维膜印迹细胞检查，操作简单，不损伤结膜，联合免疫组化染色，显微镜下观察细胞清楚，可成为检查眼表疾病的一种简便有效的辅助手段。

Application of Cellulose Acetate Membrane with the Technique of Impression Cytology Combined with Immunohistochemical Staining for Detection of Dry Eye

PURPOSE: To evaluate the conjunctival cells by impression cytology with cellulose acetate membrane. METHODS: The expression of the TGF-beta 1 in conjunctival epithelium of 24 patients with dry eye were studied by impression cytology combined with immunohistochemical staining and observed by microscope. RESULT: The cellulose acetate membrane is very transparent under the microscope. Cell's membrane by TGF-beta 1 staining is clear, cytoplasm brown and nuclei blue. CONCLUSION: The method of impression cytology with cellulose acetate membrane is simple and has no injury to the ocular surface. Combined with immunohistochemistry staining, it is suitable to observe the conjunctival cells by this technique which has an important value for the examination of the ocular surface disease.