骨髓基质细胞促进神经干细胞增殖分化

目的：探讨骨髓基质细胞（BMSCs）对神经干细胞（NSCs）增殖分化的影响。 方法:在体外比较NSCs在单独培养和在BMSCs条件培养液中培养下的分化和增殖情况。 结果:应用BMSCs条件培养液培养NSCs，分化的神经元比例较显著高于NSCs单独培养（41.1%±3.2% vs 23.3%±16.5%，P<0.05），而分化的星形胶质细胞所占比例显著降低（33.8%±4.9% vs 65.0%±10.4%，P<0.01），同时增殖细胞所占比例也显著增高（74.7％±4.7％ vs 51.4％±12.3％，P<0.01）。 结论:BMSCs对NSCs有促进其增殖和向神经元分化的作用。NSCs与BMSCs联合移植可能会增强NSCs移植的抗脑损伤作用。

Enhanced proliferation and neuronal differentiation of neural stem cells by bone marrow stromal cells

AIM:To evaluate whether bone marrow stromal cells (BMSCs) could provide a supportive microenvironment for proliferation and differentiation of neural stem cells (NSCs). METHODS:The proliferation and differentiation of NSCs were comp ared, in media without growth factors or in BMSCs-conditioned media. RESULTS:The proportion of neurons to total NSCs was significant ly increased when NSCs were cultured in BMSCs-conditioned media (41.1%±3.2% vs 23.3%±16.5%, P<0.05), while the proportion of astrocytes was sign ificantly decreased (33.8%±4.9% vs 65.0%±10.4%, P<0.01), as compa red with the NSCs cultured in media without growth factors. The proportion of pr oliferated cells was also significantly increased (74.7%±4.7% vs 51.4%± 12.3%, P<0.01). CONCLUSION:These finding indicates that BMSCs support the proliferation and neuronal differentiation of NSCs and suggests that their addition may be useful to improve outcomes of NSCs transplantation.