三七总皂苷抗心肌肥大的作用与其影响神经体液因素的关系

目的:通过研究三七总皂苷(Panax notoginseng saponins,PNS)对大鼠整体和离体模型心肌肥大的影响,探讨PNS对心肌肥大的作用机制。方法:用腹主动脉缩窄法建立大鼠整体心肌肥大模型,PNS腹腔注射给药,持续3周后,测大鼠收缩压(SBP)、全心重量/体重(HW/BW)、左室重量指数(LVI)、左室心肌纤维直径(MD);用去甲肾上腺素(NE)诱导培养的新生大鼠心肌细胞制作离体心肌肥大模型,给PNS72h后,测定细胞表面积和蛋白质含量。结果:PNS浓度依赖性地抑制腹主动脉缩窄大鼠HW/BW,LVI,MD的增加,但不能显著降低大鼠SBP;0.1-0.5g·L^-1PNS可显著抑制NE诱导的心肌细胞表面积和蛋白质含量的增加,P<0.01。结论:PNS能抑制大鼠整体和离体2种模型心肌肥大,其作用机制不是通过降低压力负荷.而是与拮抗神经体液因素NE的作用有关。

Relationship between the inhibitory effects of PNS on cardiac hypertrophy and its action on neurohormonal factor

Objective: To study the effects of total saponins of Panax notoginseng(PNS)on the overall model and vitro model of cardiac hypertrophy, and investigate the mechanism of its action. Method: Cardiac hypertrophy of rats due to pressure overload was induced by constricting of abdominal aorta. The rats were given ip PNS for three weeks after operation. Three weeks later , the systolic blood pressure (SBP), heart weight(HW), left ventricular weight(LVW), the ratio of HW/BW, LVW/BW (LVI) and the myofibril diameters(MD) in left ventricles were measured. Cardiac hypertrophy was induced by norepinephrine (NE) in cultured neonatal rat myocardial cells. PNS were given to the cultured myocardial cells,72 hours later, the cell surface area and protein content (used as the index of cardiac hypertrophy) were determined. Result: PNS inhibited the markers of cardiac hypertrophy in rats (HW/BW, LVI, MD) in a dose dependent manner, but SBP of rats wasn't obviously influenced ;0.1-0.5 g·L^-1 PNS significantly inhibited the NE induced increase of surface area and protein content in the cultured myocardial cells, P<0.01. Conclusion: PNS can prevent the overall model and vitro model of cardiac hypertrophy in rats, and this action may be related to its inhibitory action on neurohormonal factor NE,but not on pressure overload.