人类疱疹病毒7型的实验室检查初探

目的 :建立一种敏感、特异、快速诊断人类疱疹病毒 7型 (HHV- 7)的实验室检查方法。方法 :应用聚合酶链反应(PCR)和巢式聚合酶链反应 (n- PCR)对年龄 0～ 2岁正常儿童和患病儿童的吐出唾液和用无菌棉签放进口腔湿润唾液进行检测。结果 :用无菌棉签采集的口腔湿润唾液分别用 PCR和 n- PCR方法测得 HHV- 7总阳性率分别为 8.8%、6 1.6 % ,两者比较有非常显著性差异 (P <0 .0 1)。用 n- PCR方法测得吐出的唾液的总阳性率为 73% ,与同方法测得用无菌棉签采集的口腔湿润唾液标本比较 ,两者无显著性差异 (P >0 .0 5 )。结论 :用 n- PCR方法是一种临床检测 HHV- 7的快速有效手段 ,标本用无菌棉签采集的口腔湿润唾液和吐出唾液均可

Laboratory examination of human herpesvirus 7

Objective: To establish a sensitive, specific and fast method for diagnosis of HHV-7 infection. Methods: PCR and n-PCR were used to identify the infection of HHV-7. The saliva samples were selected from 0~2 years old normal and sick children.We measured their saliva spitted out or collected with sterile cotton swab .Results:The positive rate of samples collected from sterile cotton swab were 8.8% and 61.6% by using PCR and n-PCR, respectively,and the difference was significant when the two were compared with each other(P<0.01). The positive rate of saliva sample collected by spitting out was 73% by using n-PCR,with no significant difference compared with that of saliva sample from sterile cotton swab (P>0.05).Conclusion: n-PCR is a sensitive, specific method for diagnosis of HHV-7 infection.