补肾益精方延缓老年雄性大鼠骨与脑衰老的实验研究

目的 评价补肾益精方延缓老年雄性大鼠骨衰老与脑衰老的作用，探讨此方延缓整个机制衰老的机制。方法 ４０只２４月龄ＳＤ雄性大鼠随机分为２４月龄本底对照组、３０月龄增龄对照组、补肾益精方低剂量组、补肾益精方高剂量组，每组各１０只。低剂量组、高剂量组均自２４月龄给药至３０月龄。各组相应处理以后分别测定各组大鼠的骨参数（左侧股骨近段、中段、远段的骨密度和右侧股骨的弯曲断裂载荷）以及脑参数（脑组织Ｍ受体结合容

Experimental Study on Effect of Bushen Yijing Recipe in Delaying Senility of Bone and Brain of Aged Male Rats

Objective: To evaluate the effect of Bushen Yijing recipe (BSYJR) in delaying senility of bone and brain of aged male rats and infer its mechanism in delaying systemic senility. Methods: Forty male SD rats, 24 months old were randomly divided into 4 groups, the baseline control group, the aged control group (30 months old), the BSYJR high dose group and the BSYJR low dose group. The latter two groups received BSYJR treatment from 24 months old to 30 months old. Bone indexes (bone density of the proximal, middle and distal segments of left femur and break bending load of right femur) and brain indexes (binding capacity of M receptor and cholinesterase activity of brain) were measured after responding treatment. Results: In bone, BSYJR could not only increase the bone mineral density in various segments of femur, but also raise the bending break load of femur dose effect dependently. In brain, BSYJR could both up regulate the binding capacity of M receptor and inhibit the activity of cholinesterase. Conclusion: BSYJR could delay the senility of bone and brain in male rats, inferring that it might regulate integrally the abnormality of aging in Kidney Asthenia and Essence Deficiency through mediation of nerve endocrine immunity network.